{"product_id":"biolegend-320014","title":"Biolegend, 320014, Alexa Fluor® 647 anti-mouse\/rat\/human FOXP3 Antibody, 100tests","description":"\u003cp\u003eFOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + \/CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in \"scurfy\" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nReported Reactivity: Cynomolgus, Rhesus, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full-length FOXP3 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm \/ 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Roncador G, et al. 2005 Eur. J. Immunol. 35:1681. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol Yang ZZ, et al. 2006. Blood 107:3639. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. Groh V, et al. 2006. Nature Immunology 7:755. Lewkowicz P, et al. 2006 J. Immunol. 177:7155. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023. Bamias G, et al. 2007. J. Immunol. 178:1809. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed Davidson TS, et al. 2007. J. Immunol. 178:4022. MacDonald K PA, et al. 2007. Blood doi:10.1182\/blood-2007-01-067249. Jaffar Z, et al. 2007. J. Immunol. 179:6193. Müller M, et al. 2007. J. Immunol. 179:2774. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381 Klunker S, et al. 2009. J. Exp Med. PubMed Haque A, et al. 2010. J. Immunol. 184:2583. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed\u003cbr\u003e\nProduct Citations: Li S, et al. 2014. Virus Res. 180:84. PubMed Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038\/s41598-017-10135-1. PubMed Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Ou W, et al. 2023. Nat Commun. 14:392. PubMed Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed Gao R, et al. 2021. iScience. 24:103133. PubMed Chan JA, et al. 2022. Nat Commun. 13:4159. PubMed Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed Li Q, et al. 2022. Cell Rep. 40:111308. PubMed Hoang TN, et al. 2021. Cell. 184:460. PubMed Haque A, et al. 2010. J Immunol. 184:2583. PubMed Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed Kim DH, et al. 2021. eLife. 10:00. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed Colleen S McGary et al. 2017. Immunity. 47(4):776-788 . PubMed Best SA, et al. 2018. Cell Metab. 27:935. PubMed Thauland T, et al. 2014. J Immunol. 193:5894. PubMed Bamias G, et al. 2007. J Immunol. 178:1809. PubMed Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed Hoang TN, et al. 2020. bioRxiv. . PubMed Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed Frazier T, et al. 2018. Adipocyte. 0.41875. PubMed Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed Ma W, et al. 2017. Sci Rep. . 10.1038\/s41598-017-15661-6. PubMed Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed Werbner M, et al. 2019. mSystems. 4:e00292-18. PubMed Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed Yang J, et al. 2022. Microbiome. 10:149. PubMed Harper JL, et al. 2020. Nat Med. 519:26. PubMed Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed Wang Z, et al. 2015. Mol Oncol. 9: 1458-1470. PubMed He B, et al. 2020. Cell Reports. 30(3):714-724.e5.. PubMed Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed Kato H, et al. 2021. Frontiers in Immunology. 12:635531. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed Abu-El-Rub E, et al. 2020. Cell Death Dis. 0.749305556. PubMed Toriyama K, et al. 2020. Commun Biol. 0.398611111. PubMed Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed Zhang L, et al. 2021. Mol Ther. 29:744. PubMed\u003cbr\u003e\nRRID: AB_439749 (BioLegend Cat. No. 320013) AB_439750 (BioLegend Cat. No. 320014)\u003cbr\u003e\nStructure: Forkhead\/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains\u003cbr\u003e\nDistribution: Nuclear; expressed in T regulatory cells\u003cbr\u003e\nFunction: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis\u003cbr\u003e\nInteraction: Interacts with DNA\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Hori S, et al. 2003. Science 299:1057.\u003cbr\u003e\nRegulation: FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation\u003cbr\u003e\nGene ID: 2037131738250943\u003cbr\u003e\nUniProt: View information about FOXP3 on UniProt.org\u003cbr\u003e\nClone: 150D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?\u003cbr\u003e\nA: It is not recommended. It is best to use PBMCs for this testing.\u003cbr\u003e\nQ: Can FOXP3 be costained with cytokines?\u003cbr\u003e\nA: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860811305129,"sku":"320014","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-320014","provider":"Iright","version":"1.0","type":"link"}