{"product_id":"biolegend-320113","title":"Biolegend, 320113, Alexa Fluor® 647 anti-human FOXP3 Antibody, 25tests","description":"\u003cp\u003eFOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + \/CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in \"scurfy\" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Cynomolgus, Rhesus, Pigtailed Macaque\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full-length FOXP3 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm \/ 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1,8,19-20, and Western blotting1. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding. NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.(IHC) Yang ZZ, et al. 2006. Blood 107:3639. Liu W, et al. 2006. J. Exp. Med. 203:1701.PubMed Bollyky PL, et al. 2007. J. Immunol. 179:744. Bell MP, et al. 2007. J. Immunol. 179:1893. Tran DQ, et al. 2007. Blood doi:10.1182\/blood-2007-06-094656. PubMed Gao Q,et al.2007.J Clin Oncol.25:2586.(IHC) PubMed Pillai V,et al. 2008. Blood 111:463. PubMed Zheng Y, et al. 2008. J. Immunol. 181:1683. PubMed Zonios DI, et al. 2008.Blood112:287. PubMed Kavanagh B, et al. 2008. Blood. PubMed Nevala WK, et al. 2009. Clin Cancer Res. 15:1931. PubMed Grant J, et al. 2009. Cytometry B Clin Cytom. 76:69. PubMed Nigam P, et al. 2010. J. Immunol. 184:1690. PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (ICFC) PubMed Hartigan-O'Connor DJ,et al.2007.J Exp Med.204:2679. PubMed Raghaven S, et al. 2009. Ann Rheum Dis. 68:1908. PubMed Hodi FS, et al. 2014. Cancer Immunol Res. 2:632.(IHC) PubMed Sziros E, et al. 2015. Clin Cancer Res. 21:2840.(IHC) PubMed\u003cbr\u003e\nProduct Citations: Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed Xiao X, et al. 2023. Front Immunol. 14:1113303. PubMed Altorki NK, et al. 2022. Cell Rep. 39:110639. PubMed Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed Hartigan-O'Connor D, et al. 2007. J Immunol Methods. 319:41. PubMed Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Verma A, et al. 2021. Cell Rep. 37:109942. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed Weaver JD, et al. 2022. Oncoimmunology. 11:2141007. PubMed Amodio D, et al. 2018. J Immunol. 200:538. PubMed Shen X, et al. 2021. Front Immunol. 12:710750. PubMed Xu-Monette ZY, et al. 2019. Cancer Immunol Res. 7:644. PubMed Pasciuto E, et al. 2020. Cell. 182:625. PubMed Tian M, et al. 2021. Elife. 10:. PubMed Capelle CM, et al. 2022. Cell Rep Med. 3:100600. PubMed Riese P, et al. 2022. Nat Commun. 13:6894. PubMed Yang YK, et al. 2021. Stem Cell Res Ther. 12:156. PubMed Adel–Patient K, et al. 2018. Clin Transl Allergy. 8:38. PubMed Chen B, et al. 2021. Cell. 184:6262. PubMed Vanderbeke L, et al. 2021. Nat Commun. 12:4117. PubMed Jiang Q, et al. 2008. Blood. 112:2858. PubMed Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed Moysi E, et al. 2021. Front Immunol. 12:683396. PubMed\u003cbr\u003e\nRRID: AB_439753 (BioLegend Cat. No. 320113) AB_439754 (BioLegend Cat. No. 320114)\u003cbr\u003e\nStructure: Forkhead\/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains\u003cbr\u003e\nDistribution: Nuclear; expressed in T regulatory cells\u003cbr\u003e\nFunction: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis\u003cbr\u003e\nInteraction: Interacts with DNA\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Hori S, et al. 2003. Science 299:1057. 2. Gandhi R, et al. 2010. Nat. Immunol. 11:846.\u003cbr\u003e\nRegulation: FOXP3 is present at high levels in T regulatory cells, it can also be induced by T cell activation.\u003cbr\u003e\nGene ID: 50943\u003cbr\u003e\nUniProt: View information about FOXP3 on UniProt.org\u003cbr\u003e\nClone: 206D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?\u003cbr\u003e\nA: It is not recommended. It is best to use PBMCs for this testing.\u003cbr\u003e\nQ: Can FOXP3 be costained with cytokines?\u003cbr\u003e\nA: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860867535017,"sku":"320113","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-320113","provider":"Iright","version":"1.0","type":"link"}