{"product_id":"biolegend-320718","title":"Biolegend, 320718, PerCP\/Cyanine5.5 anti-human CD182 (CXCR2) Antibody, 100tests","description":"\u003cp\u003eCXCR2 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor B (IL-8RB), CD182, and CD128b. It is a CXC chemokine receptor belongs to G protein-coupled receptor (GPCR) family. CXCR2 is expressed as homodimer or heterodimer with CXCR1 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. CXCR2 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α,β,γ (CXCL1, CXCL2, CXCL3).\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CXCR2 transfected L1.2 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: The 5E8\/CXCR2 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR2 expression.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kyriakakis E, et al. 2011. J Leukoc Biol. 90:929. PubMed.\u003cbr\u003e\nProduct Citations: Jiang X, et al. 2023. Cell Mol Immunol. 1339:20. PubMed Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed Lim C, et al. 2017. Sci Rep. 10.1038\/s41598-017-11464-x. PubMed\u003cbr\u003e\nRRID: AB_2564598 (BioLegend Cat. No. 320717) AB_2564599 (BioLegend Cat. No. 320718)\u003cbr\u003e\nStructure: Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR1\u003cbr\u003e\nDistribution: Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes\u003cbr\u003e\nFunction: Neutrophil activation, chemotaxis, angiogenesis\u003cbr\u003e\nLigand\/Receptor: IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-\u0026amp; alpha;,ß, γ (CXCL1, CXCL2, CXCL3)\u003cbr\u003e\nCell Type: Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682. 2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663. 3. Emadi S, et al. 2005. Blood 105:464. 4. Omari KM, et al. 2005. Brain 128:1003. 5. Juremalm M and Nilsson G. 2005. Chem. Immunol. Allergy 87:130. 6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164.\u003cbr\u003e\nGene ID: 3579\u003cbr\u003e\nUniProt: View information about CD182 on UniProt.org\u003cbr\u003e\nClone: 5E8\/CXCR2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD182, CXCR2, CD128b, IL-8RB, Type II IL-8 receptor\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862460682409,"sku":"320718","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-320718","provider":"Iright","version":"1.0","type":"link"}