{"product_id":"biolegend-324204","title":"Biolegend, 324204, FITC anti-human CD326 (EpCAM) Antibody, 100tests","description":"\u003cp\u003eCD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: DU.4475 breast carcinoma\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Lammers R, et al. 2002. Exp. Hematol. 30:537. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed Human Protein Atlas http:\/\/www.proteinatlas.org\/ENSG00000119888\/antibody (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Zhao Q, et al. 2018. Cancer Res. 78:2370. PubMed Mair B et al. 2019. Nat Biomed Eng. 3(10):796-805 . PubMed Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed Lütge M, et al. 2023. Nat Immunol. . PubMed Vokshi BH, et al. 2023. Nat Commun. 14:3034. PubMed Chen Z, et al. 2023. Nat Commun. 14:3469. PubMed Cassandras M, et al. 2020. Nat Cell Biol. 1295:22. PubMed Peng J, et al. 2015. Cancer Res . 75: 5034 - 5045. PubMed Dong L, et al. 2020. BJU Int. 126:191. PubMed Wimberger T, et al. 2020. Bioelectrochemistry. 134:107495. PubMed Fasanya HO, et al. 2021. J Bone Oncol. 28:100357. PubMed Barnicle A, et al. 2016. Physiol Genomics. 48: 257-273. PubMed Zheng X, et al. 2020. Cancer Res. 4414:80. PubMed Garcia TM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:943. PubMed Martins Garcia T, et al. 2022. Antibiotics (Basel). 11:. PubMed Urbaniak A, et al. 2020. J Biochem Mol Toxicol. 34:e22487. PubMed Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed Jung Y, et al. 2020. Prostate. 80:1145. PubMed Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed Liang D, et al. 2022. Front Cell Dev Biol. 10:883314. PubMed Dinh PC, et al. 2020. Nat Commun. 1.197222222. PubMed Kang H, et al. 2017. PLoS One. 12(6):e0180251. PubMed Tanimizu N, et al. 2013. J Cell Sci. 126:5239. PubMed Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed McGinnis CS, et al. 2019. Nat Methods. 1.096527778. PubMed Jaganathan H, et al. 2014. PLoS One. 9:91986. PubMed Roos FJM, et al. 2021. Clin Transl Med. 11:e566. PubMed Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed Xuan Z, et al. 2020. Am J Cancer Res. 0.458333333. PubMed Roca H, et al. 2013. PLoS One. 8:76773. PubMed Hao M, et al. 2021. Developmental Cell. 56(3):341-355.e5. PubMed Hansen FJ, et al. 2022. Int J Mol Sci. 23:. PubMed Wu SZ, et al. 2020. EMBO J. 39:e104063. PubMed\u003cbr\u003e\nRRID: AB_756077 (BioLegend Cat. No. 324203) AB_756078 (BioLegend Cat. No. 324204)\u003cbr\u003e\nStructure: Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.\u003cbr\u003e\nDistribution: Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.\u003cbr\u003e\nFunction: Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.\u003cbr\u003e\nModification: Glycosylated.\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Epithelial cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Strnad J, et al. 1989. Cancer Res. 49:314. 2. Munz M, et al. 2004. Oncogene 23:5748. 3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.\u003cbr\u003e\nGene ID: 4072\u003cbr\u003e\nUniProt: View information about CD326 on UniProt.org\u003cbr\u003e\nClone: 9C4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862503575721,"sku":"324204","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-324204","provider":"Iright","version":"1.0","type":"link"}