{"product_id":"biolegend-324705","title":"Biolegend, 324705, PE anti-human erbB3\/HER-3 Antibody, 25tests","description":"\u003cp\u003eThe 1B4C3 monclonal antibody recognizes human erbB3\/HER3 also know as oncogene erbB3, tyrosine kinase cell surface receptor HER3, and C-erbB3. erbB3\/HER3 is a receptor tyrosine kinase and a member of the epidermal growth factor receptor family. erbB3\/HER3 binds the heregulin ligands and contains 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain with a predicted molecular weight approximately 148 kD. Signaling through the erbB3\/HER3 receptor induces proliferation and differentiation in some cell types, the receptor is widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. erbB3\/HER3 forms constitutively active heterodimers with erbB2\/HER2 in a subset of human mammary tumors; expression of erbB3\/HER3 reported be linked to favorable outcome in bladder and ovarian cancer. erbB3\/HER3 has been shown to interact with a number of proteins including EGF receptor, erbB2\/HER2, erbB4, Grb2, Grb7, SOS1, cdk5, SHC, FAK, PTK6, and others. The erb3\/HER3 receptor is extensively phosphorylated at residues T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, and Y1328. The 1B4C3 antibody has been shown to be useful for flow cytometric detection of human erbB3\/HER3.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: NIH-3T3 cells transfected with human erbB3\/HER3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nProduct Citations: Tiago M, et al. 2020. Br J Cancer. 122:789. PubMed Kosti P, et al. 2021. Cell Reports Medicine. 2(4):100227. PubMed Zabransky DJ, et al. 2015. Proc Natl Acad Sci U S A. 112: 6205 -6214. PubMed Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed Marrocco I, et al. 2021. EMBO Mol Med. 13:e13144. PubMed\u003cbr\u003e\nRRID: AB_756159 (BioLegend Cat. No. 324705) AB_2099569 (BioLegend Cat. No. 324706)\u003cbr\u003e\nStructure: Member of the epidermal growth factor receptor family. A receptor tyrosine kinase containing 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain. Predicted molecular weight approximately 148 kD\u003cbr\u003e\nDistribution: Widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. Forms constitutively active heterodimers with erbB2\/HER2 in a subset of human mammary tumors; expression of erbB3\/HER3 reported be linked to favorable outcome in b\u003cbr\u003e\nFunction: Induces extensive signaling cascade that can regulate growth and possibly differentiation\u003cbr\u003e\nLigand\/Receptor: Heregulin\u003cbr\u003e\nModification: Extensively phosphorylated T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, Y1328\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Carraway KL, et al. 1994. J. Biol. Chem. 269:14303. 2. Kraus MH, et al. 1989. Proc. Natl. Acad. Sci. USA 86:9193. 3. Chan SD, et al. 1995. J. Biol. Chem. 270:22608. 4. Tanner B, et al. 2006. J. Clin. Oncol. (Epub Aug. 8 2006)\u003cbr\u003e\nGene ID: 2065\u003cbr\u003e\nUniProt: View information about erbB3\/HER-3 on UniProt.org\u003cbr\u003e\nClone: 1B4C3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: erbB-3, erbB3, HER-3, HER3\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860846694569,"sku":"324705","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-324705","provider":"Iright","version":"1.0","type":"link"}