{"product_id":"biolegend-325504","title":"Biolegend, 325504, Biotin anti-human IgE Antibody, 100μg","description":"\u003cp\u003eIgE is the seventh immunoglobulin made by B cells in the immune response.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedELISA, IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.\u003cbr\u003e\nProduct Citations: Farkas AM, et al. 2019. J Allergy Clin Immunol. 143:335. PubMed Harms RZ, et al. 2015. PLoS One. e0117335:10. PubMed Mencarelli A, et al. 2024. Nat Immunol. 693:25. PubMed Li T, et al. 2018. J Biol Chem. 293:1243. PubMed\u003cbr\u003e\nRRID: AB_830848 (BioLegend Cat. No. 325503) AB_830849 (BioLegend Cat. No. 325504)\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 3497\u003cbr\u003e\nUniProt: View information about IgE on UniProt.org\u003cbr\u003e\nClone: MHE-18\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin E\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866127585449,"sku":"325504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-325504","provider":"Iright","version":"1.0","type":"link"}