{"product_id":"biolegend-328110","title":"Biolegend, 328110, PE anti-human CD90 (Thy1) Antibody, 100tests","description":"\u003cp\u003eCD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34 + cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34 + CD90 + cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: HEL cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP) Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity) Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope) Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC)\u003cbr\u003e\nProduct Citations: Kawai T, et al. 2015. Clin Cancer Res. 21: 3081 - 3091. PubMed Loegl J, et al. 2016. Reproduction. 152: 447 - 455. PubMed Zhou Y, et al. 2020. Cancer Cell. 38(6):818-828.e5. PubMed Melzer C, et al. 2020. Int J Mol Sci. :21. PubMed Korsunsky I, et al. 2022. Med. 3:481. PubMed Zhu H, et al. 2022. Cell Transplant. 31:9636897221106996. PubMed Park J, et al. 2022. Bone Joint Res. 11:777. PubMed Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed Park J, et al. 2021. Exp Ther Med. 22:808. PubMed Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed Liu C, et al. 2022. Regen Ther. 21:192. PubMed Costa MHG, et al. 2021. Biotechnol J. 16:e2000389. PubMed Labedz-Maslowska A, et al. 2021. Cells. 10: . PubMed Ye T, et al. 2023. Bioact Mater. 21:194. PubMed Liu C, et al. 2022. BMC Oral Health. 22:536. PubMed Robb KP, et al. 2022. Front Immunol. 13:972095. PubMed Tevlin R, et al. 2023. Sci Rep. 13:2097. PubMed Aru B, et al. 2023. Balkan Med J. 40:117. PubMed Goodnough LH, et al. 2020. JBMR Plus. 4:e10398. PubMed Lee JK, et al. 2020. Sci Rep. 2.567361111. PubMed Wei H, et al. 2020. Cell Death Dis. 11:290. PubMed Yang J, et al. 2022. Cell Discov. 8:92. PubMed Branco MA, et al. 2020. Methods Mol Biol. . PubMed Zhang B, et al. 2022. Stem Cells Int. 2022:2236250. PubMed Manresa MC, et al. 2020. Gastroenterology. 159:1778. PubMed Li C, et al. 2016. Cell Res. 10.1038\/cr.2016.156. PubMed Adutler-Lieber S, et al. 2013. J Cardiovasc Pharmacol Therap. 18:78. PubMed Wu S, et al. 2020. Stem Cells Int. 2019:1515040. PubMed Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed Deng CC, et al. 2021. Nat Commun. 12:3709. PubMed Samuelson C, et al. 2021. Exp Hematol. 93:52. PubMed Santos J, et al. 2013. J Transl Med. 11:18. PubMed Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed Peterson CW, et al. 2022. Mol Ther Methods Clin Dev. 24:30. PubMed Van Egeren D, et al. 2021. Cell Stem Cell. 28(3):514-523.e9. PubMed Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed Wang H, et al. 2022. Hum Gene Ther. 33:389. PubMed Ambrosi TH, et al. 2020. Aging Cell. 19:e13164. PubMed Di Stefano B, et al. 2019. Cell Stem Cell. 25:622. PubMed Alexander TB, et al. 2018. Nature. 562:373. PubMed Ye W, et al. 2022. Regen Ther. 21:477. PubMed Lance R Thurlow et al. 2018. Journal of cellular biochemistry. 120(6):9213-9229 . PubMed Morgan R, et al. 2016. PLoS One. 11: 0162008. PubMed Morris K, et al. 2014. PLoS One. 9:98656. PubMed Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed Meng Z, et al. 2022. Stem Cell Res Ther. 13:466. PubMed Lee JK, et al. 2021. Cells. 10:. PubMed Zhang Q, et al. 2021. Bone Joint Res. 10:226. PubMed Marozin S, et al. 2021. Sci Rep. 11:10933. PubMed Novak R, et al. 2022. Cell Death Dis. 13:820. PubMed Yasuda T, et al. 2021. Cell Reports. 34(8):108779. PubMed Neveu W, et al. 2015. Am J Physiol Cell Physiol. 309: C616 - C626. PubMed Sielatycka K, et al. 2017. Stem Cell Rev. 1.079166667. PubMed Wang W, et al. 2022. Int J Mol Sci. 23:. PubMed Labedz-Maslowska A, et al. 2020. Int J Mol Sci. 21:. PubMed Khan SA, et al. 2021. Cell Reports. 35(11):109233. PubMed Zhao Y, et al. 2018. Stem Cell Reports. 10:509. PubMed Wang X, et al. 2021. Inflammation. 44:1815. PubMed Di Stefano B, et al. 2018. Nat Methods. 15:732. PubMed Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed Ainsworth RI, et al. 2022. Nat Commun. 13:6221. PubMed Bailey AL, et al. 2020. bioRxiv. . PubMed Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed\u003cbr\u003e\nRRID: AB_893442 (BioLegend Cat. No. 328109) AB_893433 (BioLegend Cat. No. 328110)\u003cbr\u003e\nStructure: 25-35 kD glycoprotein, Ig superfamily\u003cbr\u003e\nDistribution: Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines\u003cbr\u003e\nFunction: Regulate hematopoiesis and neural cell growth, cell adhesion\u003cbr\u003e\nLigand\/Receptor: β3 integrin, β2 integrin\u003cbr\u003e\nCell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. McKenzie JL, et al. 1981. J. Immunol. 126:843. 2. Avalos AM, et al. 2002. Biol. Res. 35:231. 3. Wetzel A, et al. 2004. J. Immunol. 172:3850.\u003cbr\u003e\nGene ID: 7070\u003cbr\u003e\nUniProt: View information about CD90 on UniProt.org\u003cbr\u003e\nClone: 5E10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: Thy-1, Thy1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864165798057,"sku":"328110","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-328110","provider":"Iright","version":"1.0","type":"link"}