{"product_id":"biolegend-328401","title":"Biolegend, 328401, Purified anti-human CD100 Antibody, 25μg","description":"\u003cp\u003eCD100, a 150 kD homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor\/peripheral blood T cell lines, and at higher density on activated T cells and B cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human PHA activated lymphocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hall K, et al. 1996. P. Natl. Acad. Sci. USA 93:11780. Mizrahi S, et al. 2007. PLoS One. 2(9):e818. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)\u003cbr\u003e\nProduct Citations: Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed\u003cbr\u003e\nRRID: AB_1236386 (BioLegend Cat. No. 328401)\u003cbr\u003e\nStructure: Class IV semaphorin family member, 150 kD homodimer\u003cbr\u003e\nDistribution: T cells, B cells, weak on NK cells\u003cbr\u003e\nFunction: Costimulatory molecule in adaptive immune responses that switches off negative signaling from CD72\u003cbr\u003e\nCell Type: B cells, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Schlossman SL Bloumsell W Gilks et al. eds. 1995. Leucocyte Typing V:White Cell Differentiation Antigens. Oxford University Press New York. 2. Bougeret CIG Mansur H Dastot et al. 1992. Increased surface expression of a newly identified 150 kDa dimer early after\u003cbr\u003e\nGene ID: 10507\u003cbr\u003e\nUniProt: View information about CD100 on UniProt.org\u003cbr\u003e\nClone: A8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V BP383, S233\u003cbr\u003e\nOther Names: Sema4D\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862525989033,"sku":"328401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-328401","provider":"Iright","version":"1.0","type":"link"}