{"product_id":"biolegend-331201","title":"Biolegend, 331201, Purified anti-human TCR γ\/δ Antibody, 25μg","description":"\u003cp\u003eT cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α\/β) or a γ and a δ chain (TCR γ\/δ). TCR γ\/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. The γ\/δ TCR associates with CD3 and is expressed on a subset of T cells found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ\/δ T cells are CD4 - \/CD8 - , some are CD8 + . T cells expressing the γ\/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. It has been reported that γ\/δ T cells also play a principal role in antigen presentation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Pigtailed Macaque\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone B1 is also known as clone B1.1. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3 and paraffin-embedded sections5, in vitro blocking, and spatial biology (IBEX)8,9. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. Nos. 331235 and 331236).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Rodriguez-Gago M, et al. 2001. Transplantation. 72:503. Lehmann FS, et al. 2002. Am. J. Physiol. Gastrointest. Liver. Physiol. 283:G481. (FC) Bordignon M, et al. 2008. Mol. Med. Rep. 1:485. (IHC) Conrad M, et al. 2007. Cytom. Part A 71A:925. (FC) Pollinger B, et al. 2011. J. Immunol. 186:2602. (IHC) Correia DV, et al. 2011. Blood. 118:992. (Block) Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed Ataya M, et al. 2020. Am J Transplant. 20:663. PubMed Mayassi T, et al. 2019. Cell. 176:967. PubMed Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed Arunachalam PS, et al. 2021. Nature. 596:410. PubMed Zhan Y, et al. 2021. JCI Insight. 6:. PubMed Hu Y, et al. 2022. Front Oncol. 12:845765. PubMed Fenton TM, et al. 2020. Immunity. 52(3):557-570. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Ardain A, et al. 2019. Nature. 570:528. PubMed Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed Albertsson AM, et al. 2018. Am J Pathol. 188:757. PubMed Chiou SH, et al. 2021. Immunity. 54:586. PubMed\u003cbr\u003e\nRRID: AB_1089222 (BioLegend Cat. No. 331202)\u003cbr\u003e\nStructure: Ig superfamily, associates with CD3 complex\u003cbr\u003e\nDistribution: T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum\u003cbr\u003e\nFunction: Antigen recognition\u003cbr\u003e\nLigand\/Receptor: Some bacterial or tumor antigens bound MHC class I, CD1 molecule\u003cbr\u003e\nCell Type: Epithelial cells, T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Lanier LL, et al. 1987. J. Clin. Immunol. 7:429. 2. Spencer J, et al. 1989. Eur. J. Immunol. 19:1335. 3. Uyemura K, et al. 1991. J. Exp. Med. 174:683. 4. Spada FM, et al. 2000. J. Exp. Med. 191:907.\u003cbr\u003e\nGene ID: 69646965\u003cbr\u003e\nUniProt: View information about TCR gamma\/delta on UniProt.org\u003cbr\u003e\nClone: B1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell receptor γ\/δ, γ\/δ TCR, TCR-γ\/δ\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862546534569,"sku":"331201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-331201","provider":"Iright","version":"1.0","type":"link"}