{"product_id":"biolegend-337205","title":"Biolegend, 337205, PE anti-human CD11c Antibody, 25tests","description":"\u003cp\u003eCD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α x and CR4. CD11c non-covalently associates with integrin β 2 (CD18) and is expressed on monocytes\/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.75 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone Bu15 has a different binding epitope than clone 3.9. The binding of Bu15 with CD11c is divalent cation independent. Additional reported applications (for the relevant formats of this clone) include: inhibition of CD11c mediated adhesion and stimulation of chemokine production by monocytes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. Rezzonico R, et al. 2001. Blood 97:2932. Sadhu C, et al. 2007. J. Leukoc. Biol. 81:1395. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)\u003cbr\u003e\nProduct Citations: Yu B, et al. 2021. Cell. 184(7):1790-1803.e17. PubMed Polisetti N, et al. 2022. Bio Protoc. 12: . PubMed Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed Wang L, et al. 2021. Vaccines (Basel). :9. PubMed Prendergast B, et al. 2013. Brain Behav Immun. 32:104. PubMed Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed Hackstein H, et al. 2011. Cell Immunol. 271:401. PubMed Rutman AK et al. 2018. Endocrinology. 159(11):3834-3847 . PubMed\u003cbr\u003e\nRRID: AB_1236439 (BioLegend Cat. No. 337205) AB_2564135 (BioLegend Cat. No. 337224) AB_1236405 (BioLegend Cat. No. 337206)\u003cbr\u003e\nStructure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD\u003cbr\u003e\nDistribution: Myeloid, dendritic cells, NK cells, B cells and T cell subsets\u003cbr\u003e\nFunction: Adhesion, CTL killing Ligand Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 Antigen\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810.\u003cbr\u003e\nGene ID: 3687\u003cbr\u003e\nUniProt: View information about CD11c on UniProt.org\u003cbr\u003e\nClone: Bu15\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V S143\u003cbr\u003e\nOther Names: Integrin αx subunit, ITGAX, CR4, p150\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862919270569,"sku":"337205","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-337205","provider":"Iright","version":"1.0","type":"link"}