{"product_id":"biolegend-339802","title":"Biolegend, 339802, Purified anti-BrdU Antibody, 100μg","description":"\u003cp\u003eBrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.\u003cbr\u003e\n100μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested IHC-P - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Magaud J-P, et al. 1989. J. Histochem. Cytochem. 37:1517. Ejassi-Lassallette A, et al. 2011. 22:245. PubMed Ma L, et al. 2012 PNAS. 109:2090. PubMed.\u003cbr\u003e\nProduct Citations: Fu S, et al. 2023. Cells. 12:. PubMed Avalos A, et al. 2022. JCI Insight. 7: . PubMed Yu X, et al. 2023. Br J Pharmacol. 180:161. PubMed AR F, et al. 2016. Sci Rep. 6: 32900. PubMed Ejlassi-Lassallette A, et al. 2011. Mol Biol Cell. 1.086805556. PubMed Ma L, et al. 2012. Proc Natl Acad Sci U S A. 109:2090. PubMed Yoon J, et al. 2022. Cell Death Differ. . PubMed\u003cbr\u003e\nRRID: AB_1595600 (BioLegend Cat. No. 339801) AB_1595495 (BioLegend Cat. No. 339802)\u003cbr\u003e\nStructure: Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.\u003cbr\u003e\nDistribution: Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.\u003cbr\u003e\nFunction: Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Immunology\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Gratzner HG. 1982. Science 218:474. 2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. 3. Dolbeare F, et al. 1985. Cytometry 6:521.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: Bu20a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bromodeoxyuridine\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862920843433,"sku":"339802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-339802","provider":"Iright","version":"1.0","type":"link"}