{"product_id":"biolegend-339809","title":"Biolegend, 339809, Biotin anti-BrdU Antibody, 25μg","description":"\u003cp\u003eBrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.\u003cbr\u003e\n25μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Magaud J-P, et al. 1989. J. Histochem. Cytochem. 37:1517. Ejassi-Lassallette A, et al. 2011. 22:245. PubMed Ma L, et al. 2012 PNAS. 109:2090. PubMed.\u003cbr\u003e\nProduct Citations: Tsang DKL, et al. 2022. Gut Microbes. 14:2108281. PubMed Ghosh D, et al. 2020. J Cell Sci. . PubMed Anthony SM, et al. 2021. eLife. 10:00. PubMed Tomihari M, et al. 2010. Cancer Res. 70:5778. PubMed Wong E, et al. 2012. J Immunol. 189:5667. PubMed Ghosh D, et al. 2020. J Cell Sci. 133:00:00. PubMed Morita A, et al. 2018. Dev Dyn. 247:699. PubMed Jiang C, et al. 2021. Nat Commun. 1308:12. PubMed\u003cbr\u003e\nRRID: AB_1595579 (BioLegend Cat. No. 339809) AB_1595472 (BioLegend Cat. No. 339810)\u003cbr\u003e\nStructure: Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.\u003cbr\u003e\nDistribution: Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.\u003cbr\u003e\nFunction: Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Immunology\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Gratzner HG. 1982. Science 218:474. 2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. 3. Dolbeare F, et al. 1985. Cytometry 6:521.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: Bu20a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bromodeoxyuridine\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862920614057,"sku":"339809","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-339809","provider":"Iright","version":"1.0","type":"link"}