{"product_id":"biolegend-341503","title":"Biolegend, 341503, PE anti-human CCR10 Antibody, 25tests","description":"\u003cp\u003eCCR10, also known as GPR-2, is a G-protein-coupled seven transmembrane CC-chemokine receptor. It is the receptor of CCL27 (CTACK\/ALP\/ILC\/ESkine) and CCL28 (MEC) and is expressed on a small subset of T memory cells, IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts. The interaction of CCR10 with its ligands plays a role in the regulation of T cell homing into cutaneous site and IgA-secreting cells migration.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: N-terminal peptide of human CCR10\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: It has been observed that clone 6588-5 can interact with some tandem-dye antibody conjugates during multi-color staining, potentially leading to unwanted staining. These dyes include PE\/Cyanine7, PE\/Cyanine5, PE\/Dazzle™ 594, APC\/Cyanine7, APC\/Fire™ 750, and PerCP\/Cyanine5.5. This interaction can be resolved by sequentially staining with the 6588-5 antibody first and then followed by other antibodies of interest.\u003cbr\u003e\nProduct Citations: Liu RX, et al. 2023. iScience. 26:106140. PubMed Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed Jiao Q, et al. 2020. Br J Dermatol. 182:648. PubMed Sugita S, et al. 2013. Invest Ophthalmol Vis Sci . 54:6926. PubMed Qualai J, et al. 2016. PLoS One. 11: 0156605. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Kubin ME, et al. 2017. Acta Derm Venereol. 97:449. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Zamarin D, et al. 2020. Clin Cancer Res. 26:4531. PubMed\u003cbr\u003e\nRRID: AB_1595542 (BioLegend Cat. No. 341503) AB_1595446 (BioLegend Cat. No. 341504)\u003cbr\u003e\nStructure: G-protein-coupled seven transmembrane protein\u003cbr\u003e\nDistribution: Small subsets of T memory cells and IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts\u003cbr\u003e\nFunction: T cell homing into cutaneous site, regulation of IgA-secreting cells migration\u003cbr\u003e\nLigand\/Receptor: CCL27 (CTACK\/ALP\/ILC\/ESkine), CCL28 (MEC)\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Fibroblasts, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Hudak S, et al. 2002. J. Immunol. 169:1189 2. Kunkel EJ, et al. 2003. J. Clin. Invest. 111:1001 3. Homey B, et al. 2002. Nature Medicine. 8:157 4. Nakayama T, et al. 2002. J. Virol. 76:3072\u003cbr\u003e\nGene ID: 2826\u003cbr\u003e\nUniProt: View information about CCR10 on UniProt.org\u003cbr\u003e\nClone: 6588-5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: GPR-2, C-C chemokine receptor 10\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862468677801,"sku":"341503","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-341503","provider":"Iright","version":"1.0","type":"link"}