{"product_id":"biolegend-341606","title":"Biolegend, 341606, FITC anti-human CX3CR1 Antibody, 100tests","description":"\u003cp\u003eCX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes\/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CX3CR1-EGFP fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nishimura M, et al. 2002. J. Immunol. 168:6173. Nanki T, et al. 2002. Arthritis Rheum. 46:2878. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.\u003cbr\u003e\nProduct Citations: Healy LM, et al. 2020. Glia. 68:811. PubMed Benmeziane K, et al. 2022. STAR Protoc. 3:101815. PubMed Huuhtanen J, et al. 2023. J Clin Invest. 133: . PubMed Fadini G, et al. 2014. Atherosclerosis. 237:805. PubMed Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed Staumont-Sallé D, et al. 2014. J Exp Med. 211:1185. PubMed Feuser K, et al. 2012. Cytokine. 58:178. PubMed Cavarelli M, et al. 2022. iScience. 25:104346. PubMed Kim M, et al. 2017. Cytokine. 10.1016\/j.cyto.2017.07.007. PubMed Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed Hasselmann J, et al. 2020. Neuron. 103(6):1016-1033. PubMed Zhao T, et al. 2012. PLoS One. 7:e43399. PubMed Scheiblich H, et al. 2021. Cell. 184:5089. PubMed Kleinnijenhuis J, et al. 2012. Proc Natl Acad Sci U S A. 109:17537. PubMed Berg P, et al. 2012. Clin Vaccine Immunol. 1.327777778. PubMed Remmerswaal E, et al. 2012. Blood. 119:1702. PubMed Lee CH, et al. 2018. Elife. 7:e32532. PubMed Verschoor C, et al. 2014. PLoS One. 9:104522. PubMed\u003cbr\u003e\nRRID: AB_1626276 (BioLegend Cat. No. 341605) AB_1626272 (BioLegend Cat. No. 341606)\u003cbr\u003e\nStructure: G-protein-coupled seven transmembrane receptor\u003cbr\u003e\nDistribution: NK cells, T cell subset, monocytes\/macrophages, dendritic cells\u003cbr\u003e\nFunction: cell adhesion and migration\u003cbr\u003e\nLigand\/Receptor: CX3CL1 (Fractalkine, FKN, neurotactin)\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Imai T, et al. 1997. Cell. 91:521. 2. Fong AM, et al. 1998. J. Exp. Med. 188:1413. 3. Auffray C, et al. 2009. J. Exp. Med. 206:595.\u003cbr\u003e\nGene ID: 1524\u003cbr\u003e\nUniProt: View information about CX3CR1 on UniProt.org\u003cbr\u003e\nClone: 2A9-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: V28, GPR13, Chemokine C-X3-C receptor 1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866552553641,"sku":"341606","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-341606","provider":"Iright","version":"1.0","type":"link"}