{"product_id":"biolegend-347604","title":"Biolegend, 347604, PE anti-human Siglec-10 Antibody, 100tests","description":"\u003cp\u003eSiglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24 and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant Siglec-10 fused to human IgG Fc\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Munday J, et al. 2001. Biochem. J. 355:489 Yokoi H, et al. 2006. Allergy. 61:769 Kivi E, et al. 2009. Blood 114:5385 Barkal AA, et al. 2019. Nature. 572(7769):392-396. (Agonist)\u003cbr\u003e\nProduct Citations: Büll C, et al. 2016. Immunol Cell Biol. 10.1038\/icb.2016.105. PubMed Wang Z, et al. 2020. Cell Mol Immunol. 17:1148. PubMed\u003cbr\u003e\nRRID: AB_2270417 (BioLegend Cat. No. 347603) AB_2189155 (BioLegend Cat. No. 347604)\u003cbr\u003e\nStructure: Lectin, belongs to the Ig-superfamily, has one V-set Ig-like domain and three C2-set domains, a single transmembrane domain and one immunoreceptor tyrosine-based inhibitor motif (ITIM) in the cytoplasmic domain\u003cbr\u003e\nDistribution: Monocytes, eosinophils, B cells, and a subset of NK cells\u003cbr\u003e\nFunction: Cell adhesion, signal transduction\u003cbr\u003e\nInteraction: SHP-1, PTPN6\u003cbr\u003e\nLigand\/Receptor: CD24, vascular adhesion protein-1 (VAP-1)\u003cbr\u003e\nBioactivity: Inhibition of signal transduction by recruiting cytoplasmic phosphatases\u003cbr\u003e\nCell Type: B cells, Eosinophils, Monocytes, NK cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Siglec Molecules\u003cbr\u003e\nAntigen References: 1. Chen GY, et al. 2009. Science 323:1722 2. Aizawa H, et al. 2003. Genomics.82:521 3. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355\u003cbr\u003e\nGene ID: 89790\u003cbr\u003e\nUniProt: View information about Siglec-10 on UniProt.org\u003cbr\u003e\nClone: 5G6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sialic acid binding Ig-like lectin 10\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862462550185,"sku":"347604","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-347604","provider":"Iright","version":"1.0","type":"link"}