{"product_id":"biolegend-348009","title":"Biolegend, 348009, APC anti-human Granulysin Antibody, 25tests","description":"\u003cp\u003eGranulysin is a member of the saposin-like protein family of lipid binding proteins (SAPLIP). It is expressed by NK cells and cytolytic T lymphocytes (CTL) as 9 kD and 15 kD forms. The 9 kD form is derived from the 15 kD form by cleavage at both amino- and carboxy-termini. Granulysin has broad cytolytic activities against a variety of tumor cells and microbes, including Gram-positive and -negative bacteria, fungi and parasites. It also functions as a chemoattractant for inflammatory cells and activates the cells expression of cytokines and chemokines.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant 9 kD granulysin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone DH2 recognizes both the 9 kD and 15 kD isoforms of Granulysin.1ELISA Capture: To measure human granulysin, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated DH10 antibody (Cat. No. 526103) as the detection antibody. Recombinant Human Granulysin (ELISA Std.) (Cat. No. 552109) can be used as the protein standard.Flow Cytometry: The fluorochrome-labeled DH2 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 \u0026amp; 438008) are specially developed and recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hanson DA, et al. 1999. Mol. Immunol. 36:413. Spada FM, et al. 2000. J. Exp. Med. 191:937.\u003cbr\u003e\nRRID: AB_2819988 (BioLegend Cat. No. 348009) AB_2819989 (BioLegend Cat. No. 348010)\u003cbr\u003e\nStructure: Saposin-like protein family of lipid binding protein, 9 kD and 15 kD\u003cbr\u003e\nDistribution: NK cells, cytolytic T cells\u003cbr\u003e\nFunction: Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Krensky AM. 2000. Biochem. Pharmacol. 59:317. 2. Deng A, et al. 2005. J. Immunol. 174:5243. 3. Kaspar AA, et al. 2001. J. Immunol. 167:350. 4. Krensky AM and Clayberger C. 2009. Tissue Antigens 73:193.\u003cbr\u003e\nGene ID: 10578\u003cbr\u003e\nUniProt: View information about Granulysin on UniProt.org\u003cbr\u003e\nClone: DH2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863009022121,"sku":"348009","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-348009","provider":"Iright","version":"1.0","type":"link"}