{"product_id":"biolegend-350305","title":"Biolegend, 350305, PE anti-human CD1d Antibody, 25tests","description":"\u003cp\u003eCD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes\/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to i NKT cells analogous to MHC molecule presentation of peptides to T cells.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Cynomolgus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CD1d-Fc fusion\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Exley M, et al. 2000. Immunology 100:37. (IHC, WB) Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB) Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)\u003cbr\u003e\nProduct Citations: Loh L, et al. 2024. Cell Rep. 114705:43. PubMed\u003cbr\u003e\nRRID: AB_10642028 (BioLegend Cat. No. 350305) AB_10641845 (BioLegend Cat. No. 350306)\u003cbr\u003e\nStructure: MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily\u003cbr\u003e\nDistribution: Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells\u003cbr\u003e\nFunction: Present lipid antigens to iNKT cells\u003cbr\u003e\nLigand\/Receptor: When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, MHC Antigens, TCRs\u003cbr\u003e\nAntigen References: 1. Koch M, et al. 2005. Nat. Immunol. 6:819. 2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010. 3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889. 4. Teige A, et al. 2010. J. Immunol. 185:345.\u003cbr\u003e\nGene ID: 912\u003cbr\u003e\nUniProt: View information about CD1d on UniProt.org\u003cbr\u003e\nClone: 51.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: R3G1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862428897449,"sku":"350305","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-350305","provider":"Iright","version":"1.0","type":"link"}