{"product_id":"biolegend-351321","title":"Biolegend, 351321, PerCP\/Cyanine5.5 anti-human CD127 (IL-7Rα) Antibody, 25tests","description":"\u003cp\u003eCD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human CD127\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for the relevant formats) application: proteogenomics1.\u003cbr\u003e\nAdditional Product Notes: View more applications data for this product in our Scientific Poster Library.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)\u003cbr\u003e\nProduct Citations: Yan YN, et al. 2023. Mediators Inflamm. 2023:8215567. PubMed Côrte-Real BF, et al. 2022. Front Immunol. 13:1005965. PubMed Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed Roukens AHE, et al. 2022. Nat Immunol. 23:23. PubMed Wu J, et al. 2021. Front Immunol. 12:601611. PubMed Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed Goodwin M, et al. 2020. Sci Adv. 6:eaaz0571. PubMed Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed Shen C, et al. 2021. Front Immunol. 12:680055. PubMed OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed Van Zeebroeck L, et al. 2021. Front Immunol. 12:655122. PubMed Santoni de Sio FR, et al. 2018. J Allergy Clin Immunol. 142:1909. PubMed Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed\u003cbr\u003e\nRRID: AB_10900253 (BioLegend Cat. No. 351321) AB_10897104 (BioLegend Cat. No. 351322)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD\u003cbr\u003e\nDistribution: Immature B cells through early pre-B stage, thymocytes (except CD4\/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells\u003cbr\u003e\nFunction: T cell and immature B cell proliferation and development\u003cbr\u003e\nLigand\/Receptor: IL-7\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701.\u003cbr\u003e\nGene ID: 3575\u003cbr\u003e\nUniProt: View information about CD127 on UniProt.org\u003cbr\u003e\nClone: A019D5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-7 receptor α chain, IL-7Rα\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864382492841,"sku":"351321","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-351321","provider":"Iright","version":"1.0","type":"link"}