{"product_id":"biolegend-353214","title":"Biolegend, 353214, APC anti-human CD197 (CCR7) Antibody, 100tests","description":"\u003cp\u003eCCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CCR7-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nProduct Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Prockop SE, et al. 2023. J Clin Invest. 133:. PubMed Li M, et al. 2021. J Clin Invest. 131:. PubMed Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed Andonian BJ, et al. 2022. Sci Rep. 12:7450. PubMed Shen Y, et al. 2021. Cell Stress Chaperones. 26:937. PubMed Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed Blengio F, et al. 2013. Immunobiology. 218:76. PubMed Fajgenbaum DC, et al. 2019. J Clin Invest. 130:4451. PubMed Liu C, et al. 2021. Cell Res. 31:1106. PubMed Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed Jo T, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed Dai X, et al. 2021. Bioact Mater. 1.659027778. PubMed Cho JH, et al. 2021. Nat Commun. 12:792. PubMed Soriano-Sarabia N, et al. 2014. J Virol. 88:14070. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Bradley T et al. 2018. Cell reports. 25(1):107-117 . PubMed Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed Ronaghan NJ, et al. 2022. PLoS One. 17:e0276013. PubMed Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed\u003cbr\u003e\nRRID: AB_10915474 (BioLegend Cat. No. 353213) AB_10917387 (BioLegend Cat. No. 353214)\u003cbr\u003e\nStructure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.\u003cbr\u003e\nDistribution: T cells, B cells, NK, dendritic cells.\u003cbr\u003e\nFunction: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.\u003cbr\u003e\nLigand\/Receptor: CCL19 and CCL21.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed\u003cbr\u003e\nGene ID: 1236\u003cbr\u003e\nUniProt: View information about CD197 on UniProt.org\u003cbr\u003e\nClone: G043H7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR2, CDw197, EBI1, CMKBR7\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866096947369,"sku":"353214","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-353214","provider":"Iright","version":"1.0","type":"link"}