{"product_id":"biolegend-353220","title":"Biolegend, 353220, PerCP\/Cyanine5.5 anti-human CD197 (CCR7) Antibody, 100tests","description":"\u003cp\u003eCCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CCR7-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nProduct Citations: Zhang S, et al. 2015. J Virol. 89: 8304-8317. PubMed Liu B, et al. 2015. J Virol. 89: 11834 - 11844. PubMed Henault J, et al. 2015. Nat Immunol. 17: 196-203. PubMed Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed Ye L, et al. 2022. Cell Metab. 34:595. PubMed Kwiecie? I, et al. 2020. Cells. 9:00. PubMed Weiskopf D, et al. 2015. J Virol. 89:120. PubMed Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed Chiarini M, et al. 2012. Mult Scler. 1.297222222. PubMed Karim F, et al. 2021. Elife. 10:. PubMed Anft M, et al. 2021. J Nephrol. 6:. PubMed Rutkowska E, et al. 2021. Cells. 10:. PubMed Arlehamn C, et al. 2016. PLoS Pathog. 12: 1005760. PubMed Jensen S, et al. 2015. PLoS One. 10: e0139573. PubMed Weiskopf D, et al. 2015. Proc Natl Acad Sci U S A. 112: E4256-E4263. PubMed Arlehamn C, et al. 2014. J Immunol. 193:2931. PubMed Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed Seo IH, et al. 2021. Immune Netw. 21:e17. PubMed Démoulins T, et al. 2014. Nanomedicine. 10:1739. PubMed Wang J, et al. 2020. Cancer Immunol Res. 794:8. PubMed Ferrer-Font L, et al. 2021. Curr Protoc. 1:e222. PubMed Seumois G, et al. 2016. J Immunol. 197: 655 - 664. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Jung JH, et al. 2021. Nat Commun. 12:4043. PubMed Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed Bhattacharya P, et al. 2020. Elife. 9:00. PubMed Hurton L, et al. 2016. Proc Natl Acad Sci U S A. 113: E7788 - E7797. PubMed Lundy SK, et al. 2018. Front Med (Lausanne). 0.38125. PubMed Nakachi S, et al. 2017. Arthritis Research \u0026amp; Therapy . 10.1186\/s13075-017-1309-x. PubMed Burel JG, et al. 2019. Elife. 8. PubMed Thieme CJ, et al. 2020. Cell Rep Med. 1:100092. PubMed Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed Jensen S, et al. 2015. PLoS One. 10: 0145249. PubMed Seo IH, et al. 2021. Cell Reports. 36(4):109438. PubMed Delmas A, et al. 2016. PLoS One. 11: 0163305. PubMed Alwis R, et al. 2016. J Virol. 90: 4771 - 4779. PubMed Kwiecien I, et al. 2022. Front Immunol. 13:864497. PubMed Havenith S, et al. 2012. Int Immunol. 24:625. PubMed Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed\u003cbr\u003e\nRRID: AB_10915275 (BioLegend Cat. No. 353219) AB_10916121 (BioLegend Cat. No. 353220)\u003cbr\u003e\nStructure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.\u003cbr\u003e\nDistribution: T cells, B cells, NK, dendritic cells.\u003cbr\u003e\nFunction: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.\u003cbr\u003e\nLigand\/Receptor: CCL19 and CCL21.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed\u003cbr\u003e\nGene ID: 1236\u003cbr\u003e\nUniProt: View information about CD197 on UniProt.org\u003cbr\u003e\nClone: G043H7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR2, CDw197, EBI1, CMKBR7\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866098323625,"sku":"353220","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-353220","provider":"Iright","version":"1.0","type":"link"}