{"product_id":"biolegend-353225","title":"Biolegend, 353225, PE\/Cyanine7 anti-human CD197 (CCR7) Antibody, 25tests","description":"\u003cp\u003eCCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CCR7-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nProduct Citations: Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed Guo A, et al. 2022. Nature. 607:135. PubMed Li M, et al. 2023. Front Immunol. 14:1087923. PubMed Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed Eberhardt F, et al. 2023. Int J Mol Med. 52:. PubMed Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed Mayassi T, et al. 2019. Cell. 176:967. PubMed Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed Ishizaka A, et al. 2016. J Virol. 90: 5665 - 5676. PubMed Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed Huang X, et al. 2021. Nat Nanotechnol. 16:214. PubMed Gruber T, et al. 2020. JCI Insight. 5:00. PubMed Claireaux M, et al. 2018. MBio. 9:e00317. PubMed Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed Schmidt J, et al. 2021. Cell Rep Med. 100194:2. PubMed Stephenson E, et al. 2021. Nat Med. 27:904. PubMed Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed Mukhopadhyay M, et al. 2017. J Immunol. 10.4049\/jimmunol.1700953. PubMed Swanson E, et al. 2021. eLife. 10:00. PubMed Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed Nakasone H, et al. 2021. Commun Biol. 4:1177. PubMed Sefik E, et al. 2021. Nat Biotechnol. . PubMed Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed Gyurdieva A, et al. 2022. Nat Commun. 13:5296. PubMed Chellappa S, et al. 2016. J Leukoc Biol. 100: 5 - 16. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Pillet S, et al. 2016. Clin Immunol. 168:72-87. PubMed Rakshit S, et al. 2020. Cell Rep. 33:108451. PubMed Gannon PO, et al. 2020. Cytotherapy. 22(12):780-791. PubMed Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed Savage AK, et al. 2021. iScience. 24(5):102404. PubMed Valle-Casuso JC, et al. 2019. Cell Metab. 29:611. PubMed Michaels K, et al. 2015. J Immunol. 194:3267. PubMed Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed Genge PC, et al. 2021. STAR Protoc. 2:100900. PubMed Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed Gannon P, et al. 2015. J Immunol. 195: 356 - 366. PubMed Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed Siedlik J, et al. 2017. J Immunol Methods. 10.4049\/jimmunol.1700003. PubMed Li M, et al. 2020. Nat Commun. 4051:11. PubMed Fu J, et al. 2021. J Clin Invest. 131:. PubMed Martins AJ, et al. 2017. Cell Systems. 4(4):379-392.e12. PubMed Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed\u003cbr\u003e\nRRID: AB_11125576 (BioLegend Cat. No. 353225) AB_11126145 (BioLegend Cat. No. 353226)\u003cbr\u003e\nStructure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.\u003cbr\u003e\nDistribution: T cells, B cells, NK, dendritic cells.\u003cbr\u003e\nFunction: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.\u003cbr\u003e\nLigand\/Receptor: CCL19 and CCL21.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed\u003cbr\u003e\nGene ID: 1236\u003cbr\u003e\nUniProt: View information about CD197 on UniProt.org\u003cbr\u003e\nClone: G043H7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR2, CDw197, EBI1, CMKBR7\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866098028713,"sku":"353225","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-353225","provider":"Iright","version":"1.0","type":"link"}