{"product_id":"biolegend-354001","title":"Biolegend, 354001, Purified anti-human Tim-4 Antibody, 25μg","description":"\u003cp\u003eThe T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th-1 and Th-2) cell-mediated immunity. Tim-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of Tim-1. This binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, Tim-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The Tim-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and is connected to Tim-1-triggered Th2 responsiveness.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human TIM-4- IgV Fc, fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 9F4 recognizes the FG loop of human Tim-41.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kobayashi N, et al. 2007. Immunity 27:927.\u003cbr\u003e\nProduct Citations: De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed\u003cbr\u003e\nRRID: AB_11126746 (BioLegend Cat. No. 354002)\u003cbr\u003e\nStructure: Type 1 transmembrane glycoprotein and mucin domain containing protein 4.\u003cbr\u003e\nDistribution: Splenic CD14+, macrophages, immature DC.\u003cbr\u003e\nFunction: Tim-1 ligation to Tim-4 induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.\u003cbr\u003e\nLigand\/Receptor: Phosphatiylserine is the receptor of Tim-4, and Tim-4 is a ligand of Tim-1.\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nAntigen References: 1. Kobayashi N, et al. 2007. Immunity 27:927. 2. Meyers JH, et al. 2005. Nat. Immunol. 6:455. 3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454. 4. Shakhov AN, et al. 2004. Eur. J. Immunol. 34:494.\u003cbr\u003e\nGene ID: 91937\u003cbr\u003e\nUniProt: View information about Tim-4 on UniProt.org\u003cbr\u003e\nClone: 9F4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862513963177,"sku":"354001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-354001","provider":"Iright","version":"1.0","type":"link"}