{"product_id":"biolegend-355607","title":"Biolegend, 355607, APC anti-human CD227 (MUC-1) Antibody, 25tests","description":"\u003cp\u003eMucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1\/SOS1\/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2\/neu.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Jurkat cells transfected with MUC1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: This clone shows stronger binding to the glycosylated compared to the non-glycosylated peptide1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Song W, et al. 2012. Int. J Oncol. 41:1977. PubMed\u003cbr\u003e\nProduct Citations: Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed Wang J, et al. 2022. Front Immunol. 13:863346. PubMed Norgard RJ, et al. 2021. EMBO Rep. 22:e51872. PubMed Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed\u003cbr\u003e\nRRID: AB_2565311 (BioLegend Cat. No. 355607) AB_2565312 (BioLegend Cat. No. 355608)\u003cbr\u003e\nStructure: 500-1000 kD proteoglycan (glycosylated form)\u003cbr\u003e\nDistribution: Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers\u003cbr\u003e\nInteraction: Complexes with SOS1 and Grb2 in RAS signaling\u003cbr\u003e\nLigand\/Receptor: ICAM-1 and Her2\/neu (ErbB2)\u003cbr\u003e\nCell Type: Dendritic cells, Epithelial cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Gendler SJ. 2001. J. Mammary Gland Biol. Neoplasia. 6:339. 2. Agrawal B, et al. 1998. Cancer Res. 58:4079. 3. Rahn JJ, et al. 2004. J. Biol. Chem. 279:29386.\u003cbr\u003e\nGene ID: 4582\u003cbr\u003e\nUniProt: View information about CD227 on UniProt.org\u003cbr\u003e\nClone: 16A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Mucin-1, MUC-1, Episialin, HMFG antigen, MAM6\u003cbr\u003e\nIsotype: Mouse IgG1, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860817825961,"sku":"355607","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-355607","provider":"Iright","version":"1.0","type":"link"}