{"product_id":"biolegend-356901","title":"Biolegend, 356901, Purified anti-human CD185 (CXCR5) Antibody, 25μg","description":"\u003cp\u003eCD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CXCR5-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Law JC, et al. 2021. J Immunol. 206:37. PubMed Spoerl S, et al. 2021. Eur J Immunol. 51:1436. PubMed Lu X, et al. 2021. J Exp Med. 218: . PubMed Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed Lartey S, et al. 2020. J Infect Dis. 221:21. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed de Jong SE, et al. 2021. Nature Immunology. 22(5):654-665. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Schulten V, et al. 2018. J Allergy Clin Immunol. 141:775. PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Majri SS, et al. 2018. J Immunol. 200:110. PubMed\u003cbr\u003e\nRRID: AB_2561810 (BioLegend Cat. No. 356901) AB_2561811 (BioLegend Cat. No. 356902)\u003cbr\u003e\nStructure: G-protein coupled receptor, 7 transmembrane regions, 42 kD\u003cbr\u003e\nDistribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.\u003cbr\u003e\nFunction: Mediates cell migration to the B cell follicles in the secondary lymphoid organs\u003cbr\u003e\nLigand\/Receptor: CXCL13\u003cbr\u003e\nCell Type: B cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241. 2. León B, et al. 2012. Nat. Immunol. 13:681. 3. Crotty S. 2011. Annu. Rev. Immunol. 29:621. 4. Kerfoot SM, et al. 2011. Immunity 34:947. 5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.\u003cbr\u003e\nGene ID: 643\u003cbr\u003e\nUniProt: View information about CD185 on UniProt.org\u003cbr\u003e\nClone: J252D4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR1, MDR15\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866112938153,"sku":"356901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-356901","provider":"Iright","version":"1.0","type":"link"}