{"product_id":"biolegend-357304","title":"Biolegend, 357304, PE anti-human Ganglioside GD2 Antibody, 100tests","description":"\u003cp\u003eGanglioside GD2 is a sialic acid-containing glycosphingolipid involved in cell attachment to the extracellular matrix. Expression of GD2 in normal tissue is restricted to cells from the central nervous system, peripheral nerves, skin melanocytes, and mesenchymal stem cells. However GD2 is highly expressed by tumors of neuro-ectodermal origin such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma. GD2 has been proposed as a marker for some cancer stem cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Neuroblastoma cell line LAN-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone 14G2a is an isotype switch variant from parental hybridoma 14.18 (IgG3)1. Additional reported applications (for the relevant formats) include: inducing apoptosis and enhancing cytotoxicity of chemotherapeutic drugs in the neuroblastoma cell line 2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for use in cytotoxic and apoptotic studies (contact our custom solutions team). This clone has also been published as 14.G2a.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Mujoo K, et al. 1989. Cancer Res. 49:2857. (Cyt) Kowalczyk A, et al. 2009. Cancer Lett. 281:171. (Apop, Cyt) Battula VL, et al. 2012. J. Clin. Invest. 122:2066. (FC)\u003cbr\u003e\nProduct Citations: Baniel CC, et al. 2020. J Immunother Cancer. 8:00. PubMed Baniel CC, et al. 2020. Front Immunol. 1.576388889. PubMed Brignole C, et al. 2021. J Exp Clin Cancer Res. 40:180. PubMed Richman SA, et al. 2018. Cancer Immunol Res. 6:36. PubMed Fisher J, et al. 2017. Mol Ther. 10.1016\/j.ymthe.2017.03.002. PubMed Morris ZS, et al. 2018. Cancer Immunol Res. 6:825. PubMed Barton J, et al. 2019. F1000Res. 8:823. PubMed Strobel SB, et al. 2021. Life (Basel). 11:. PubMed Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed Voeller J, et al. 2019. J Immunother Cancer. 0.530555556. PubMed Magee K, et al. 2021. PLoS One. 16:e0255798. PubMed\u003cbr\u003e\nRRID: AB_2561884 (BioLegend Cat. No. 357303) AB_2561885 (BioLegend Cat. No. 357304)\u003cbr\u003e\nDistribution: Neurons, mesenchymal stem cells, highly expressed in tumors of neuro-ectodermal origin, such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma\u003cbr\u003e\nFunction: Cell attachment to extracellular matrix\u003cbr\u003e\nCell Type: Mesenchymal cells, Neurons\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Adhesion, Cell Biology, Immunology, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules\u003cbr\u003e\nAntigen References: 1. Tarek N, et al. 2012. J. Clin. Invest. 122:3260. 2. Matthay KK, et al. 2012. Clin. Cancer Res. 18:2740. 3. Navid F, et al. 2010. Curr. Cancer Drug Targets. 10:200.\u003cbr\u003e\nGene ID: 2583\u003cbr\u003e\nUniProt: View information about Ganglioside GD2 on UniProt.org\u003cbr\u003e\nClone: 14G2a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Disialoganglioside GD2, Ganglioside G2\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860807209129,"sku":"357304","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-357304","provider":"Iright","version":"1.0","type":"link"}