{"product_id":"biolegend-357310","title":"Biolegend, 357310, Biotin anti-human Ganglioside GD2 Antibody, 100μg","description":"\u003cp\u003eGanglioside GD2 is a sialic acid-containing glycosphingolipid involved in cell attachment to the extracellular matrix. Expression of GD2 in normal tissue is restricted to cells from the central nervous system, peripheral nerves, skin melanocytes, and mesenchymal stem cells. However GD2 is highly expressed by tumors of neuro-ectodermal origin such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma. GD2 has been proposed as a marker for some cancer stem cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Neuroblastoma cell line LAN-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 14G2a is an isotype switch variant from parental hybridoma 14.18 (IgG3)1. Additional reported applications (for the relevant formats) include: inducing apoptosis and enhancing cytotoxicity of chemotherapeutic drugs in the neuroblastoma cell line 2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for use in cytotoxic and apoptotic studies (contact our custom solutions team). This clone has also been published as 14.G2a.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Mujoo K, et al. 1989. Cancer Res. 49:2857. (Cyt) Kowalczyk A, et al. 2009. Cancer Lett. 281:171. (Apop, Cyt) Battula VL, et al. 2012. J. Clin. Invest. 122:2066. (FC)\u003cbr\u003e\nProduct Citations: Coleman CB, et al. 2018. J Virol. :92. PubMed\u003cbr\u003e\nRRID: AB_2800973 (BioLegend Cat. No. 357310)\u003cbr\u003e\nDistribution: Neurons, mesenchymal stem cells, highly expressed in tumors of neuro-ectodermal origin, such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma\u003cbr\u003e\nFunction: Cell attachment to extracellular matrix\u003cbr\u003e\nCell Type: Mesenchymal cells, Neurons\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Adhesion, Cell Biology, Immunology, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules\u003cbr\u003e\nAntigen References: 1. Tarek N, et al. 2012. J. Clin. Invest. 122:3260. 2. Matthay KK, et al. 2012. Clin. Cancer Res. 18:2740. 3. Navid F, et al. 2010. Curr. Cancer Drug Targets. 10:200.\u003cbr\u003e\nGene ID: 2583\u003cbr\u003e\nUniProt: View information about Ganglioside GD2 on UniProt.org\u003cbr\u003e\nClone: 14G2a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Disialoganglioside GD2, Ganglioside G2\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860807143593,"sku":"357310","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-357310","provider":"Iright","version":"1.0","type":"link"}