{"product_id":"biolegend-357407","title":"Biolegend, 357407, APC anti-human CD4 Antibody, 25tests","description":"\u003cp\u003eCD4, also known as T4\/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes\/macrophages. CD4 is part of the TCR\/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human CD4 T cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nProduct Citations: Lafôrets F, et al. 2023. iScience. 26:106514. PubMed Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed Ringelstein-Harlev S, et al. 2023. Eur J Haematol. 110:371. PubMed Sun L, et al. 2023. Mol Med Rep. 27: . PubMed Angiari S, et al. 2020. Cell Metab. 31:391. PubMed Feng R, et al. 2021. Scand J Immunol. 93:e13007. PubMed Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Zhan Y, et al. 2021. JCI Insight. 6:. PubMed Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed Liu T, et al. 2022. Front Immunol. 13:901349. PubMed Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed Feng Y, et al. 2022. Life (Basel). 12:. PubMed Cheng J, et al. 2021. Biomed Res Int. 2021:6614784. PubMed Lin G, et al. 2021. Mol Med Rep. 23:. PubMed Danzer H, et al. 2020. Elife. 9:00. PubMed Zheng Z, et al. 2021. Biomed Res Int. 2021:5535578. PubMed Yang JY, et al. 2020. J Cell Mol Med. . PubMed Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed\u003cbr\u003e\nRRID: AB_2565659 (BioLegend Cat. No. 357407) AB_2565660 (BioLegend Cat. No. 357408)\u003cbr\u003e\nStructure: 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily\u003cbr\u003e\nDistribution: Helper T cells, subset of thymocytes, monocytes\/macrophages, type II NKT cells\u003cbr\u003e\nInteraction: Part of the TCR\/CD3 complex\u003cbr\u003e\nLigand\/Receptor: β2 domain from the MHC class II\u003cbr\u003e\nCell Type: T cells, Monocytes, Macrophages, NKT cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557.\u003cbr\u003e\nGene ID: 920\u003cbr\u003e\nUniProt: View information about CD4 on UniProt.org\u003cbr\u003e\nClone: A161A1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T4\/Leu-3\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.\u003cbr\u003e\nA: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA\/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864402317481,"sku":"357407","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-357407","provider":"Iright","version":"1.0","type":"link"}