{"product_id":"biolegend-358511","title":"Biolegend, 358511, PE\/Cyanine7 anti-human\/mouse Bcl-6 Antibody, 25tests","description":"\u003cp\u003eB-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to T FH . This molecule is also expressed in some B cell lymphomas and breast cancer cells.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Sequence 226-398 of murine BCL6 fused with GST\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting1,2,3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Vikstrom, et al. 2010. Science 330:1095. (WB) Kallies A, et al. 2011. Blood 117:1869. (WB) Lüthje K, et al. 2012. Nat. Immunol. 13:491. (WB) Tonti E, et al. 2012. J. Immunol. 188:3217. (ICFC)\u003cbr\u003e\nProduct Citations: Koers J, et al. 2023. Front Immunol. 13:1082154. PubMed Kim CJ, et al. 2018. Immunity. 49:1034. PubMed Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed Frasca D, et al. 2018. PLoS One. 13:e0197472. PubMed\u003cbr\u003e\nRRID: AB_2566195 (BioLegend Cat. No. 358511) AB_2566196 (BioLegend Cat. No. 358512)\u003cbr\u003e\nStructure: Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers\u003cbr\u003e\nDistribution: Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells\u003cbr\u003e\nFunction: Transcription factor, master regulator of germinal center reaction\u003cbr\u003e\nInteraction: BLIMP-1\u003cbr\u003e\nCell Type: B cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Basso K and Dalla-Favera R. 2012. Immunol. Rev. 247:172. 2. Vinuesa CG, and Cyster JG, 2011. Immunity 35:671. 3. Kitano M, et al. 2011. Immunity 34:961. 4. Baumjohann D, et al. 2011. J. Immunol. 187:2089. 5. Johnston RJ, et al. 2009. Science. 325:1006. 6. Tran TH, et al. 2010. Cancer Res. 70:1711.\u003cbr\u003e\nGene ID: 604\u003cbr\u003e\nUniProt: View information about Bcl-6 on UniProt.org\u003cbr\u003e\nClone: 7D1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B-cell lymphoma 6, BCL5, LAZ3, BCL6A, ZNF51, ZBTB27\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862486274217,"sku":"358511","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-358511","provider":"Iright","version":"1.0","type":"link"}