{"product_id":"biolegend-358916","title":"Biolegend, 358916, Biotin anti-human CD199 (CCR9) Antibody, 100μg","description":"\u003cp\u003eHuman CD199, also known as CCR9, is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in the gut-associated immune response. It is highly expressed on different stages of thymocytes and upregulated on CD4 + CD8 + cells. Expression of CCR9 on γ\/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and the generation of gut-specific immunological memory.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Cells transfected with human CCR9\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2715884 (BioLegend Cat. No. 358916)\u003cbr\u003e\nStructure: A member of G-protein coupled receptors which contains seven transmembrane domains. Human CCR9 has above 80% amino acid sequence homology with its mouse counterpart.\u003cbr\u003e\nDistribution: CD4+CD8+ thymocytes, γ\/δ T cells, subset of memory T cells\u003cbr\u003e\nFunction: May direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory; co-receptor for HIV-1\u003cbr\u003e\nLigand\/Receptor: CCL25\u003cbr\u003e\nCell Type: T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Zaballos A, et al. 1999. J. Immunol. 162:5671. 2. Wurbel MA, et al. 2007. J. Immunol. 178:7598. 3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.\u003cbr\u003e\nGene ID: 10803\u003cbr\u003e\nUniProt: View information about CD199 on UniProt.org\u003cbr\u003e\nClone: L053E8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: GPR 9-6, GPR28, CDw199, Chemokine Receptor 9\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866116116649,"sku":"358916","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-358916","provider":"Iright","version":"1.0","type":"link"}