{"product_id":"biolegend-361105","title":"Biolegend, 361105, PE anti-human\/mouse\/rat MR1 Antibody, 25tests","description":"\u003cp\u003eMHC-related protein 1, also known as MR1, is a nonclassical MHC class I molecule highly conserved in mammals, particularly in its α1\/α2 domains. MR1 is required for the development and expansion of the mucosal-associated invariant T (MAIT) cells. The transcripts for MR1 are ubiquitously expressed, but its expression on the cell surface seems to be ligand-dependent. MR1 is involved in the presentation of vitamin-derived compounds from bacterial biosynthetic pathways.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nReported Reactivity: Cow\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Soluble complexes of MR1 ectodomain and B2M.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: blocking MR1-dependent activation of MAIT hybridomas, clones, or MAIT TCR-transduced cell lines1-6, detection of folded MR1 conformers3,7, and immunoprecipitation of MR1 from cell lysates7.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Young MH, et al. 2013. PLoS One. 8:e53789. (Block) Leeansyah E, et al. 2013. Blood 121:1124. (Block) Kjer-Nielsen L, et al. 2012. Nature 491:717. (Block, ELISA) Reantragoon R, et al. 2012. J. Exp. Med. 209:761. (Block, FC) Gold MC, et al. 2010. PLoS Biol. 8:e1000407. (Block, FC) Huang S, et al. 2009. Proc. Natl. Acad. Sci. USA 106:8290. (Block, FC) Huang S, et al. 2005. J. Biol. Chem. 280:21183. (IP, ELISA, FC)\u003cbr\u003e\nProduct Citations: Souter MNT, et al. 2022. J Exp Med. 219:. PubMed Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed McSharry BP, et al. 2020. Cell Reports. 30(9):2948-2962. PubMed Tang JS, et al. 2022. Front Immunol. 13:946713. PubMed Laugel B, et al. 2016. J Immunol. 197: 971 - 982. PubMed\u003cbr\u003e\nRRID: AB_2563042 (BioLegend Cat. No. 361105) AB_2563043 (BioLegend Cat. No. 361106)\u003cbr\u003e\nStructure: Member of the MHC class I family, 1 Ig-like C1-type domain, forms heterodimer with B-2 Microglobulin.\u003cbr\u003e\nDistribution: MR1 transcripts are ubiquitously expressed, but its expression in the cell surface seems to be ligand-dependent.\u003cbr\u003e\nFunction: Antigen presentation, required for development and expansion of the mucosal-associated invariant T (MAIT) cells.\u003cbr\u003e\nLigand\/Receptor: α-mannosyl ceramide derivatives, 6-formyl pterin, derivatives from bacterial riboflavin biosynthetic pathway).\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Gold MC and Lewinsohn DM. 2013. Nat. Rev. Microbiol. 11:14. 2. Young MH and Gapin L. 2013. Cell Res. 23:460. 3. Gold MC, et al. 2013. Mucosal Immunol. 6:35. 4. Meierovics A, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E3119.\u003cbr\u003e\nGene ID: 31401506425119\u003cbr\u003e\nUniProt: View information about MR1 on UniProt.org\u003cbr\u003e\nClone: 26.5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Major Histocompatibility Complex Class I-related, HLALS\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860881133737,"sku":"361105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-361105","provider":"Iright","version":"1.0","type":"link"}