{"product_id":"biolegend-361502","title":"Biolegend, 361502, Purified anti-human HLA-DQ Antibody, 100μg","description":"\u003cp\u003eHLA-DQ is heterodimeric cell surface glycoprotein comprised of a 27 kD α (heavy) chain and a 32 kD β (light) chain. In contrast to other MHC class II molecules, both chains of HLA-DQ are polymorphic and the α chain shows a high degree of polymorphism. It is expressed on B cells, activated T cells, monocytes\/macrophages, dendritic cells, and other non-professional APCs. In hematopoietic development, HLA-DR and DP are expressed first, followed by HLA-DQ. Variations in the HLA gene expression are crucial to graft survival.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human PBL\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Tü169 has been reported to react with HLA-DQ1 and HLA-DQ2. It also shows weak reactivity to HLA-DQ3.Additional reported applications (for the relevant formats) include: immunofluorescence2 and in vitro blocking1. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Benagiano M, et al. 2012. Proc. Natl. Acad. Sci. USA 109:1222. (Block) Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF) Nasi A, et al. 2013. J. Immunol. 191:3090. (FC) Turrini R, et al. 2011. Cancer Immunol. Immunother. 60:1639. (FC)\u003cbr\u003e\nProduct Citations: Chang SH, et al. 2019. Exp Mol Med. 51:28. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed\u003cbr\u003e\nRRID: AB_2563137 (BioLegend Cat. No. 361502)\u003cbr\u003e\nStructure: Ig superfamily, MHC class II, heterodimeric transmembrane protein\u003cbr\u003e\nDistribution: B cells, activated T cells, monocytes\/macrophages, dendritic cells, other APCs\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Thorsby E. 1974. Transplant. Rev. 18:51. 2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207. 3. Servenius B, et al. 1984. EMBO J. 3:3209. 4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105. 5. Strassmann G, et al. 1985. Hum. Immunol. 13:125. 6. Trowsdale J, et al. 1985. Immunol. Rev. 85:5. 7. Dorman J, et al. 2000. Epidemiologic Reviews 22:218. 8. van Lith M, et al. 2010. J. Biol. Chem. 285:40800.\u003cbr\u003e\nGene ID: 31173119\u003cbr\u003e\nUniProt: View information about HLA-DQ on UniProt.org\u003cbr\u003e\nClone: Tü169\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864063594665,"sku":"361502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-361502","provider":"Iright","version":"1.0","type":"link"}