{"product_id":"biolegend-361712","title":"Biolegend, 361712, APC\/Fire™ 750 anti-human HLA-DR, DP, DQ Antibody, 100tests","description":"\u003cp\u003eHLA-DR, HLA-DP, and HLA-DQ are heterodimeric cell surface glycoproteins comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes\/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3\/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Cynomolgus, Cat, Dog, Cow\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human PBL\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC\/Fire™ 750 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * APC\/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Tü39 has been reported to react with a shared epitope of HLA-DR, HLA-DP, and HLA-DQ. Additional reported applications (of relevant formats) include immunoprecipitation6, in vitro blocking of MLR5, and suppressor cell generation4. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pawelec G, et al. 1985. Hum. Immunol. 12:165. Shaw S, et al. 1985. Hum. Immunol. 12:191. Ziegler A, et al. 1986. Immunobiology 171:77. Pawelec G, et al. 1986. Hum. Immunol. 17:343. (Block) Dai Z, et al. 2009. J. Exp. Med. 206:793. (Block) Pawelec G, et al. 1988. J. Exp. Med. 167:243. (IP)\u003cbr\u003e\nRRID: AB_2750313 (BioLegend Cat. No. 361711) AB_2750314 (BioLegend Cat. No. 361712)\u003cbr\u003e\nStructure: Ig superfamily, MHC class II, heterodimeric transmembrane protein\u003cbr\u003e\nDistribution: B cells, activated T cells, monocytes\/macrophages, dendritic cells, other APCs\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Thorsby E. 1974. Transplant. Rev. 18:51. 2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207. 3. Servenius B, et al. 1984. EMBO J. 3:3209. 4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105. 5. Strassmann G, et al. 1985. Hum. Immunol. 13:125. 6. Trowsdale J, et al. 1985. Immunol Rev. 85:5.\u003cbr\u003e\nGene ID: 312231233113311531173119\u003cbr\u003e\nUniProt: View information about HLA-DR DP DQ on UniProt.org\u003cbr\u003e\nClone: Tü39\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864065036457,"sku":"361712","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-361712","provider":"Iright","version":"1.0","type":"link"}