{"product_id":"biolegend-367104","title":"Biolegend, 367104, PE anti-human CD14 Antibody, 100tests","description":"\u003cp\u003eCD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified human peripheral blood monocytes.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028. Devitt A, et al. 1998. Nature 6675:505.\u003cbr\u003e\nProduct Citations: Lee PY, et al. 2020. Journal of Allergy and Clinical Immunology. 146(5):1194-1200.e1. PubMed Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Piede N, et al. 2023. Cells. 12:. PubMed Laine A, et al. 2023. Microorganisms. 11:. PubMed Wu J, et al. 2022. Nat Commun. 13:676. PubMed Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed Du Y, et al. 2022. FASEB J. 36:e22248. PubMed Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed Karantanos T, et al. 2022. Haematologica. :. PubMed Bediaga NG, et al. 2022. Diabetes. 71:566. PubMed Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed Wang H, et al. 2022. Front Immunol. 13:852436. PubMed Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed Gärtner K, et al. 2018. Cancer Med. 11:39. PubMed Terenzi DC, et al. 2021. STAR Protoc. 2:100311. PubMed Ugawa M, et al. 2021. Elife. 10:. PubMed Hu H, et al. 2022. Exp Ther Med. 24:487. PubMed Keenan CR, et al. 2020. Blood. 135:2049. PubMed Aagaard K, et al. 2017. Sci Rep. 7:41389. PubMed Bediaga NG, et al. 2021. Sci Rep. 0.825. PubMed Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed Chen R, et al. 2021. Front Oncol. 11:743050. PubMed\u003cbr\u003e\nRRID: AB_2565887 (BioLegend Cat. No. 367103) AB_2565888 (BioLegend Cat. No. 367104)\u003cbr\u003e\nStructure: GPI-linked membrane glycoprotein.\u003cbr\u003e\nDistribution: Monocytes, macrophages, dendritic cells, and granulocytes.\u003cbr\u003e\nFunction: LPS receptor, clearance of Gram-negative pathogens.\u003cbr\u003e\nInteraction: LPS.\u003cbr\u003e\nLigand\/Receptor: LPS.\u003cbr\u003e\nCell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: Stocks SC, et al. 1990. Biochem. J. 268:275. Wright SD, et al. 1990. Science 4975:1431.\u003cbr\u003e\nGene ID: 929\u003cbr\u003e\nUniProt: View information about CD14 on UniProt.org\u003cbr\u003e\nClone: 63D3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862467072169,"sku":"367104","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-367104","provider":"Iright","version":"1.0","type":"link"}