{"product_id":"biolegend-367140","title":"Biolegend, 367140, Brilliant Violet 711™ anti-human CD14 Antibody, 100tests","description":"\u003cp\u003eCD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified human peripheral blood monocytes.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710\/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028. Devitt A, et al. 1998. Nature 6675:505.\u003cbr\u003e\nProduct Citations: Sarhan D, et al. 2022. iScience. 25:105317. PubMed\u003cbr\u003e\nRRID: AB_2814322 (BioLegend Cat. No. 367139) AB_2814323 (BioLegend Cat. No. 367140)\u003cbr\u003e\nStructure: GPI-linked membrane glycoprotein.\u003cbr\u003e\nDistribution: Monocytes, macrophages, dendritic cells, and granulocytes.\u003cbr\u003e\nFunction: LPS receptor, clearance of Gram-negative pathogens.\u003cbr\u003e\nInteraction: LPS.\u003cbr\u003e\nLigand\/Receptor: LPS.\u003cbr\u003e\nCell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: Stocks SC, et al. 1990. Biochem. J. 268:275. Wright SD, et al. 1990. Science 4975:1431.\u003cbr\u003e\nGene ID: 929\u003cbr\u003e\nUniProt: View information about CD14 on UniProt.org\u003cbr\u003e\nClone: 63D3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862468415657,"sku":"367140","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-367140","provider":"Iright","version":"1.0","type":"link"}