{"product_id":"biolegend-368510","title":"Biolegend, 368510, PE anti-human CD45 Antibody, 100tests","description":"\u003cp\u003eCD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human PBMC\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33. Csiba A, et al. 1984. Br. J. Cancer 50:699. Tchilian EZ, et al. 2001. J. Immunol. 166:1308. Lee MS, et al. 2004. Int. Immunol. 16:1109.\u003cbr\u003e\nProduct Citations: Cillo AR, et al. 2022. Clin Cancer Res. 28:4968. PubMed Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed Chang L, et al. 2023. Life Sci Alliance. 6:. PubMed Nisa BU, et al. 2023. Life (Basel). 13:. PubMed Seree-Aphinan C, et al. 2023. Front Immunol. 14:1138765. PubMed Molpeceres C, et al. 2023. Int J Bioprint. 9:720. PubMed Wu L, et al. 2023. Cell Res. . PubMed Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed Sasamoto Y, et al. 2022. Cell Rep. 40:111166. PubMed Ding L, et al. 2023. J Clin Invest. 133: . PubMed Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed Sluzalska K,et al. 2017. Sci Rep.. 10.1038\/s41598-017-14004-9. PubMed Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed Dam T, et al. 2021. Front Physiol. 11:613367. PubMed Sluzalska KD, et al. 2017. Osteoarthritis Cartilage. 25:1890. PubMed Zhuang J, et al. 2020. Nucleic Acids Res. 48:8870. PubMed Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed Zhang X, et al. 2022. Nat Commun. 13:5211. PubMed Li Y, et al. 2021. Aging (Albany NY). 13:25886. PubMed Chan JM, et al. 2021. Cancer Cell. 39:1479. PubMed Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed Liu Y, et al. 2022. iScience. 25:104405. PubMed Yu C, et al. 2020. Sci Rep. 10:14521. PubMed Yu LC, et al. 2020. Front Neurol. 1.104166667. PubMed Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed Rai MA, et al. 2020. Retrovirology. 17:35. PubMed Quintanal-Villalonga A, et al. 2022. STAR Protoc. 3:101776. PubMed Tidu F, et al. 2021. iScience. 24(6):102683. PubMed Zhang Y, et al. 2018. Stem Cells Int. 2018:7159465. PubMed Chioh FW, et al. 2021. eLife. 10:00. PubMed Yao X, et al. 2022. Bioeng Transl Med. 7:e10250. PubMed Zhang H et al. 2018. Cell. 175(5):1244-1258 . PubMed Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed Dong N, et al. 2022. World J Stem Cells. 14:556. PubMed Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed Boya M, et al. 2022. Nat Commun. 13:3385. PubMed\u003cbr\u003e\nRRID: AB_2566369 (BioLegend Cat. No. 368509) AB_2566370 (BioLegend Cat. No. 368510)\u003cbr\u003e\nStructure: Type I transmembrane protein, 180 - 240 kD (multiple isoforms)\u003cbr\u003e\nDistribution: Hematopoietic cells, except circulating erythrocytes or platelets.\u003cbr\u003e\nFunction: Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, and CD4.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Protein Kinases\/Phosphatase, TCRs\u003cbr\u003e\nAntigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.\u003cbr\u003e\nGene ID: 5788\u003cbr\u003e\nUniProt: View information about CD45 on UniProt.org\u003cbr\u003e\nClone: 2D1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Leukocyte Common Antigen (LCA), T200\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862304182441,"sku":"368510","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-368510","provider":"Iright","version":"1.0","type":"link"}