{"product_id":"biolegend-369504","title":"Biolegend, 369504, Alexa Fluor® 647 anti-ERK1\/2 Phospho (Thr202\/Tyr204) Antibody, 100tests","description":"\u003cp\u003eERK1\/2 are members of mitogen-activated kinases (MAPKs) of serine\/threonine protein kinases. ERK1\/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1\/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone 6B8B69 was found to strongly cross-react with mouse ERK1\/2 Phospho (Thr202\/Tyr204) when tested in-house on C57BL\/6 mouse splenocytes.\u003cbr\u003e\nProduct Citations: Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed\u003cbr\u003e\nRRID: AB_2571895 (BioLegend Cat. No. 369503) AB_2571896 (BioLegend Cat. No. 369504)\u003cbr\u003e\nStructure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.\u003cbr\u003e\nDistribution: Cytoplasm and the nucleus.\u003cbr\u003e\nFunction: ERK1\/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.\u003cbr\u003e\nInteraction: ERK1\/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Postsynaptic proteins, Phospho-Proteins, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.\u003cbr\u003e\nGene ID: 5594\u003cbr\u003e\nUniProt: View information about ERK1\/2 on UniProt.org\u003cbr\u003e\nClone: 6B8B69\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: P44\/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864376135849,"sku":"369504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-369504","provider":"Iright","version":"1.0","type":"link"}