{"product_id":"biolegend-371105","title":"Biolegend, 371105, Alexa Fluor® 647 anti-human Aiolos Antibody, 25tests","description":"\u003cp\u003eAiolos, also known as Ikaros family zinc finger 3 (IKZF3), is a 58kD zinc finger protein involved in hematopoetic-specific transcriptional activation. Aiolos is involved in regulation of gene expression in B lymphocytes through chromatin remodeling and formation of homodimers and heterodimers with other members of the Ikaros family. Deletion of Aiolos has been reported in pre-B-acute lymphocytic leukemia (B-ALL).\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nProduct Citations: Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed\u003cbr\u003e\nRRID: AB_2572171 (BioLegend Cat. No. 371105) AB_2572172 (BioLegend Cat. No. 371106)\u003cbr\u003e\nStructure: 58kD, zinc finger protein.\u003cbr\u003e\nDistribution: T and B lymphocytes.\u003cbr\u003e\nFunction: Hematopoetic-specific transcriptional activator and chromatin remodeler.\u003cbr\u003e\nInteraction: Forms heterodimers with Ikaros family members IKZF4 and IKZF5.\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Chromatin Remodeling\/Epigenetics, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319. 2. Ma S, et al. 2008. Blood. 111:1396. 3. Billot K, et al. 2010. Leuk. Res. 34:289. 4. Morgan B, et al. 2004. EMBO J. 16:2004. 5. Wang JH, et al. 1998. Immunity. 9:543-53. 6. Hosokawa Y, et al. 1999. Genomics. 61:326.\u003cbr\u003e\nGene ID: 22806\u003cbr\u003e\nUniProt: View information about Aiolos on UniProt.org\u003cbr\u003e\nClone: 16D9C97\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IKZF3, AIO, ZNFN\/A3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864360079529,"sku":"371105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-371105","provider":"Iright","version":"1.0","type":"link"}