{"product_id":"biolegend-371305","title":"Biolegend, 371305, PE anti-human CD99 Antibody, 25tests","description":"\u003cp\u003eCD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human thymus\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Waclavicek M, et al. 1998. J. Immunol. 161:4671. Pickl W, et al. 2001. J. Virol. 75:7175.\u003cbr\u003e\nProduct Citations: Yeremenko N, et al. 2013. Arthritis Rheum. 174:65. PubMed de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed\u003cbr\u003e\nRRID: AB_2616973 (BioLegend Cat. No. 371305) AB_2616974 (BioLegend Cat. No. 371306)\u003cbr\u003e\nStructure: Surface glycoprotein, type I transmembrane protein, 32kD.\u003cbr\u003e\nDistribution: T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.\u003cbr\u003e\nFunction: Leukocyte migration, T-cell adhesion, T-cell death- caspase independent, and spontaneous rosette formation with erythrocytes.\u003cbr\u003e\nInteraction: Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.\u003cbr\u003e\nCell Type: Leukemia, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Gelin C, et al. 1989. EMBO. 8:3253. 2. Goodfellow PJ, et al. 1986. Science 234:740. 3. Pettersen RD, et al. 2001. J. Immunol. 166:4931.\u003cbr\u003e\nGene ID: 4267\u003cbr\u003e\nUniProt: View information about CD99 on UniProt.org\u003cbr\u003e\nClone: 3B2\/TA8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD99, MIC2, HBA71, MSK5X, E2 antigen\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862386626729,"sku":"371305","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-371305","provider":"Iright","version":"1.0","type":"link"}