{"product_id":"biolegend-372902","title":"Biolegend, 372902, Purified anti-human CD8a Antibody, 100μg","description":"\u003cp\u003eCD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a\/a) or heterodimer (CD8a\/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α 3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of the alpha chain of the human CD8 molecule.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality testedActiv, FC, IHC-F, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include activation3, flow cytometry4, immunohistochemical staining of frozen tissue sections2, and Western blotting5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88. (IHC-P) Petukhova L, et al. 2010. Nature 466:113. (IHC-F) Clement M, et al. 2011. J. Immunol. 187:654. (Activ) MarTchal R, et al. 2010. BMC Cancer 10:340. (FC) Pontiggia L, et al. 2008. J. Invest. Dermatol. 129:480. (WB)\u003cbr\u003e\nProduct Citations: Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed Mokhtari Z, et al. 2023. Front Oncol. 13:1069696. PubMed Nickl V, et al. 2023. Cancers (Basel). 15:. PubMed Zhang W, et al. 2022. Dev Cell. 57:329. PubMed Wen J, et al. 2020. J Pathol Clin Res. 0.36875. PubMed Giadans CG, et al. 2019. J Viral Hepat. 26:727. PubMed Zheng Y, et al. 2022. iScience. 25:103785. PubMed Rye IH, et al. 2022. Mol Oncol. 16:88. PubMed Schwabenland M, et al. 2021. Immunity. . PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed\u003cbr\u003e\nRRID: AB_2650657 (BioLegend Cat. No. 372902)\u003cbr\u003e\nStructure: Ig superfamily, homodimer or heterodimer with CD8β.\u003cbr\u003e\nDistribution: Majority of thymocytes, T cell subset, NK cells.\u003cbr\u003e\nFunction: MHC class I co-receptor, thymic differentiation, T-cell activation.\u003cbr\u003e\nLigand\/Receptor: MHC class I molecules.\u003cbr\u003e\nCell Type: NK cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88.\u003cbr\u003e\nGene ID: 100387674\u003cbr\u003e\nUniProt: View information about CD8a on UniProt.org\u003cbr\u003e\nClone: C8\/144B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T8, Leu2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862952628393,"sku":"372902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-372902","provider":"Iright","version":"1.0","type":"link"}