{"product_id":"biolegend-374202","title":"Biolegend, 374202, Purified anti-human CD86 Antibody, 100μg","description":"\u003cp\u003eCD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes\/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: ARH 77 (B lymphoblastoid cell line).\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested Blocking, IP, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Ji T, et al. 2023. Oncogenesis. 12:31. PubMed Ceprián N, et al. 2021. Front Med (Lausanne). 8:705159. PubMed Song S, et al. 2021. Commun Biol. 4:1338. PubMed Hu G, et al. 2021. Nat Commun. 12:773. PubMed\u003cbr\u003e\nRRID: AB_2687347 (BioLegend Cat. No. 374202)\u003cbr\u003e\nStructure: Ig superfamily, single-chain transmembrane glycoprotein, 80 kD\u003cbr\u003e\nDistribution: Monocytes\/macrophages, activated B cells and T cells, dendritic cells\u003cbr\u003e\nFunction: T Cell activation\u003cbr\u003e\nInteraction: C-Jun, NFκB\u003cbr\u003e\nLigand\/Receptor: CD28, CD152\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Hathcock K, et al. 1996. Adv. Immunol. 62:131. 2. June C, et al. 1994. Immunol. Today 15:321.\u003cbr\u003e\nGene ID: 942\u003cbr\u003e\nUniProt: View information about CD86 on UniProt.org\u003cbr\u003e\nClone: BU63\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: B7-2, B70, Ly-58\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862925430953,"sku":"374202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-374202","provider":"Iright","version":"1.0","type":"link"}