{"product_id":"biolegend-376808","title":"Biolegend, 376808, PE\/Cyanine7 anti-human CD45RO Antibody, 100tests","description":"\u003cp\u003eCD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes\/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic human CD45 peptide spanning the junction region between exons 3 and 7.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The S19021B antibody can be used in combination with antibodies against CD45RA to discern memory and naïve T cells. Staining profile by S19021B on human peripheral blood lymphocyte is comparable to that by clone UCHL1. Staining signal by clone S19021B on target cells can be completely blocked by pre-incubation with clone UCHL1; staining of UCHL1 on target cells can be partially blocked by pre-incubation with clone S19021B. Staining signal by clone S19021B can be significantly increased on the cells after neuraminidase treatment, suggesting that S19021B binding epitope is likely affected by glycosylation.\u003cbr\u003e\nRRID: AB_3083368 (BioLegend Cat. No. 376807) AB_3083368 (BioLegend Cat. No. 376808)\u003cbr\u003e\nStructure: Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)\u003cbr\u003e\nDistribution: Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes\u003cbr\u003e\nFunction: Enhances TCR and BCR signaling\u003cbr\u003e\nCell Type: B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Thomas M. 1989. Annu Rev Immunol. 7:339. Trowbridge I, et al. 1994. Annu Rev Immunol. 12:85.\u003cbr\u003e\nGene ID: 5788\u003cbr\u003e\nUniProt: View information about CD45RO on UniProt.org\u003cbr\u003e\nClone: S19021B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PTPRC, CD45R, B220, T200, GP180\u003cbr\u003e\nIsotype: Mouse IgG1, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866262524073,"sku":"376808","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-376808","provider":"Iright","version":"1.0","type":"link"}