{"product_id":"biolegend-387510","title":"Biolegend, 387510, PE\/Cyanine7 anti-human CD275 (B7-H2, ICOSL) Antibody, 100tests","description":"\u003cp\u003eB7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes\/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human B7-H2 recombinant protein (19-258 aa)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone W21036I completely blocks clone 9F.8A4 but does not block clone 2D3.\u003cbr\u003e\nRRID: AB_3699042 (BioLegend Cat. No. 387509) AB_3699042 (BioLegend Cat. No. 387510)\u003cbr\u003e\nStructure: Ig superfamily, B7 family, 40 kD\u003cbr\u003e\nDistribution: B cells and dendritic cells\u003cbr\u003e\nFunction: Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production\u003cbr\u003e\nInteraction: B cells, Dendritic cells\u003cbr\u003e\nLigand\/Receptor: ICOS\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Wang S, et al. 2002. J Biol Chem. 96:2808. Wong SC, et al. 2003. Blood. 102:1831.\u003cbr\u003e\nGene ID: 23308\u003cbr\u003e\nUniProt: View information about CD275 on UniProt.org\u003cbr\u003e\nClone: W21036I\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866428231849,"sku":"387510","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-387510","provider":"Iright","version":"1.0","type":"link"}