{"product_id":"biolegend-387604","title":"Biolegend, 387604, PE anti-human G-CSF Antibody, 100tests","description":"\u003cp\u003eHuman G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrades important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes\/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro , G-CSF displays strong antiapoptotic activity in neuronal cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant Human G-CSF\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clones A20052D and A20052Q do not block each other.\u003cbr\u003e\nRRID: AB_2941577 (BioLegend Cat. No. 387603) AB_2941577 (BioLegend Cat. No. 387604)\u003cbr\u003e\nStructure: Heterodimer\u003cbr\u003e\nDistribution: Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, epithelial cells, and bone marrow stromal cells\u003cbr\u003e\nFunction: G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxido anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.\u003cbr\u003e\nInteraction: Myeloid progenitor cells\u003cbr\u003e\nLigand\/Receptor: CD114 (G-CSFR)\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Nagata S, et al. 1986. Nature. 319:415-8. Kim HK, et al. 2006. Blood. 108:812-20. Donahue RE, et al. 2009. Blood. 114:2530-41. Shimoji K, et al. 2010. Cell Stem Cell. 6:227-37. Martins A, et al. 2010. IUBMB Life. 62:611-7. Hara M, et al. 2011. J Exp Med. 208:715-27.\u003cbr\u003e\nGene ID: 1441\u003cbr\u003e\nUniProt: View information about G-CSF on UniProt.org\u003cbr\u003e\nClone: A20052Q\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granulocyte-Colony Stimulating Factor, CSF3, CSF-β\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866069422249,"sku":"387604","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-387604","provider":"Iright","version":"1.0","type":"link"}