{"product_id":"biolegend-394803","title":"Biolegend, 394803, PE anti-human CD289 (TLR9) Antibody, 25tests","description":"\u003cp\u003eTLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CD289 (TLR9)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC, FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: S16013D clone can be used for both surface and intracellular detection of TLR9. ICFC compatible with both the intracellular flow cytometric staining and True-Nuclear™ transcription buffer set. Does not work for WB (tested on Daudi cell line).\u003cbr\u003e\nProduct Citations: Chen X, et al. 2023. Cancer Immunol Res. 150:11. PubMed\u003cbr\u003e\nRRID: AB_2801037 (BioLegend Cat. No. 394803) AB_2801038 (BioLegend Cat. No. 394804)\u003cbr\u003e\nDistribution: TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).\u003cbr\u003e\nLigand\/Receptor: TLR9 recognizes unmethylated CpG sequences in DNA.\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Innate Immune Signaling, Toll Like Receptors\u003cbr\u003e\nAntigen References: Hornung V, et al. 2002. J. Immunol. 168:4531. Eaton-Bassiri A, et al. 2004. Infect. Immun. 72:7202. Krieg, A. 2007. J. Clin. Invest. 117:1184.\u003cbr\u003e\nGene ID: 54106\u003cbr\u003e\nUniProt: View information about CD289 on UniProt.org\u003cbr\u003e\nClone: S16013D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD289, TLR9, Toll-like receptor 9\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866223268009,"sku":"394803","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-394803","provider":"Iright","version":"1.0","type":"link"}