{"product_id":"biolegend-396405","title":"Biolegend, 396405, PE anti-human\/mouse Granzyme B Recombinant Antibody, 25tests","description":"\u003cp\u003eGranzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL\/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).\u003cbr\u003e\nProduct Citations: Gao X, et al. 2023. Front Immunol. 14:1142428. PubMed Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed Lee W, et al. 2021. J Virol. 95:e0053021. PubMed\u003cbr\u003e\nRRID: AB_2801074 (BioLegend Cat. No. 396405) AB_2801075 (BioLegend Cat. No. 396406)\u003cbr\u003e\nStructure: 32 kD serine protease\u003cbr\u003e\nDistribution: Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells\u003cbr\u003e\nFunction: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA\/ IL-2 and CD4+ T lymphocytes with anti CD3\/CD28 or CD3\/CD46.\u003cbr\u003e\nInteraction: Caspase-3\u003cbr\u003e\nLigand\/Receptor: Mannose-6-phosphate receptor\u003cbr\u003e\nCell Type: Neutrophils, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience\u003cbr\u003e\nMolecular Family: Enzymes and Regulators, Proteases\u003cbr\u003e\nAntigen References: Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. Wagner C, et al. 2008. Mol. Immunol. 45:1761.\u003cbr\u003e\nGene ID: 300214939\u003cbr\u003e\nUniProt: View information about Granzyme B on UniProt.org\u003cbr\u003e\nClone: QA18A28\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866181062825,"sku":"396405","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-396405","provider":"Iright","version":"1.0","type":"link"}