{"product_id":"biolegend-396413","title":"Biolegend, 396413, Brilliant Violet 421™ anti-human\/mouse Granzyme B Recombinant Antibody, 25tests","description":"\u003cp\u003eGranzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL\/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).\u003cbr\u003e\nProduct Citations: Wang F, et al. 2023. Int J Mol Sci. 24:. PubMed Lin CH, et al. 2023. iScience. 26:106904. PubMed Yang QC, et al. 2023. iScience. 26:106916. PubMed Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed Wu J, et al. 2023. Bioact Mater. 20:259. PubMed Liang Y, et al. 2022. Theranostics. 12:7729. PubMed Xie Z, et al. 2022. Cell Discov. 8:133. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Peng W, et al. 2023. Cells. 12: . PubMed Toomer G, et al. 2022. Viruses. 14:. PubMed Duan H, et al. 2021. J Clin Invest. 131:. PubMed Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed Wilson TL, et al. 2022. Cancer Discov. 12:2098. PubMed Wang F, et al. 2021. Neoplasia. 23:281. PubMed\u003cbr\u003e\nRRID: AB_2810602 (BioLegend Cat. No. 396413) AB_2810603 (BioLegend Cat. No. 396414)\u003cbr\u003e\nStructure: 32 kD serine protease\u003cbr\u003e\nDistribution: Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells\u003cbr\u003e\nFunction: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA\/ IL-2 and CD4+ T lymphocytes with anti CD3\/CD28 or CD3\/CD46.\u003cbr\u003e\nInteraction: Caspase-3\u003cbr\u003e\nLigand\/Receptor: Mannose-6-phosphate receptor\u003cbr\u003e\nCell Type: Neutrophils, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience\u003cbr\u003e\nMolecular Family: Enzymes and Regulators, Proteases\u003cbr\u003e\nAntigen References: Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. Wagner C, et al. 2008. Mol. Immunol. 45:1761.\u003cbr\u003e\nGene ID: 300214939\u003cbr\u003e\nUniProt: View information about Granzyme B on UniProt.org\u003cbr\u003e\nClone: QA18A28\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866181914793,"sku":"396413","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-396413","provider":"Iright","version":"1.0","type":"link"}