{"product_id":"biolegend-400687","title":"Biolegend, 400687, PE\/Fire™ 700 Rat IgG2b, κ Isotype Ctrl Antibody, 25μg","description":"\u003cp\u003eThe isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.\u003cbr\u003e\n25μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Trinitrophenol + KLH\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Fire™ 700 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Fire™ 700 has a maximum excitation of 650 nm and a maximum emission of 700 nm.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Cervantes-Barragan L, et al. 2007. Blood 109:1131. Zeiser R, et al. 2007. Blood 109:2225. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed Yi H, et al. 2009. Blood 113:5819. PubMed Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed Lei GS, et al. 2015. Infect Immun. 83:572. PubMed Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed\u003cbr\u003e\nRRID: AB_3097106 (BioLegend Cat. No. 400687) AB_3097106 (BioLegend Cat. No. 400688)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: RTK4530\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866212421801,"sku":"400687","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-400687","provider":"Iright","version":"1.0","type":"link"}