{"product_id":"biolegend-400702","title":"Biolegend, 400702, Purified Rat IgG2c, κ Isotype Ctrl Antibody, 500μg","description":"\u003cp\u003eThe isotype of RTK4174 immunoglobulin is rat IgG2c, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.\u003cbr\u003e\n500μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Trinitrophenol + KLH\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The immunoglobulin was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICFC, ICC, IHC, IP, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.\u003cbr\u003e\nApplication Notes: The RTK4174 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The ULEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400732-400737) as negative control.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ganeshah K, et al. 2012. J. Immunol. 188:594. PubMed D'Agostino PM, et al. 2012. PNAS. 109:6175. PubMed\u003cbr\u003e\nProduct Citations: Maor-Nof M, et al. 2013. Cell Rep. 971:3. PubMed D'Agostino P, et al. 2012. Proc Natl Acad Sci U S A. 109:6175. PubMed Ray HC, et al. 2020. Sci Rep. 10:15808. PubMed Prideaux M, et al. 2021. Sci Rep. 11:22593. PubMed Bryce K 2012. J Immunol. 188:594. PubMed\u003cbr\u003e\nRRID: AB_326567 (BioLegend Cat. No. 400701) AB_326568 (BioLegend Cat. No. 400702)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: RTK4174\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Rat IgG2c, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866209865897,"sku":"400702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-400702","provider":"Iright","version":"1.0","type":"link"}