{"product_id":"biolegend-401411","title":"Biolegend, 401411, Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl Antibody, 5mg","description":"\u003cp\u003eThe isotype of MG1-45 immunoglobulin is mouse IgG1, κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.\u003cbr\u003e\n5mg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Trinitrophenol + KLH\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/µg of the protein (\u0026lt; 0.001 ng\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. Older lots may have also been bottled at 1 mg\/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC, ICFC - Quality tested WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.\u003cbr\u003e\nApplication Notes: The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401404) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 401408) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ozeki M, et al. 2008. J. Leukocyte Biol. 84:769. PubMed Kraetzel K, et al. 2008. Eur. Respair. J. 32:563. PubMed de Jong MA, et al. 2008. J Gen Virol. 89:2398. PubMed Romee R, et al. 2012 Blood. 120:4751. PubMed. Wong-Baeza I, et al. 2013. J. Immunol. 190:3216. PubMed.\u003cbr\u003e\nProduct Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Chen F, et al. 2020. Cancer Med. 9:3584. PubMed Gabriely G, et al. 2021. iScience. 24:103347. PubMed Ma YV, et al. 2021. MAbs. 13:2003281. PubMed Berg V, et al. 2020. Immunohorizons. 0.28125. PubMed Lerrer S, et al. 2021. iScience. 24:103020. PubMed White JM, et al. 2020. Mol Pharm. 1139:17. PubMed Vazquez Rodriguez G et al. 2018. Frontiers in immunology. 1.602083333 . PubMed Vazquez Rodriguez G, et al. 2017. Cancer Immunol Res. 10.1158\/2326-6066.CIR-16-0150. PubMed Gravastrand C, et al. 2017. Acta Biomaterialia. 10.1016\/j.actbio.2017.05.052. PubMed Chou CC, et al. 2022. MAbs. 14:2029675. PubMed Chen J, et al. 2021. J Invest Dermatol. 787:141. PubMed Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed Gimpel AK, et al. 2022. Front Immunol. 12:761816. PubMed\u003cbr\u003e\nRRID: AB_11148722 (BioLegend Cat. No. 401407) AB_11148942 (BioLegend Cat. No. 401408) AB_11148942 (BioLegend Cat. No. 401411) AB_11148942 (BioLegend Cat. No. 401412) AB_11148942 (BioLegend Cat. No. 401413) AB_11148942 (BioLegend Cat. No. 401414)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: MG1-45\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Mouse (BALB\/c) IgG1, κ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866125750441,"sku":"401411","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-401411","provider":"Iright","version":"1.0","type":"link"}