{"product_id":"biolegend-405151","title":"Biolegend, 405151, Purified Streptavidin, 10mg","description":"\u003cp\u003eStreptavidin is a 52 kD tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol\/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.\u003cbr\u003e\n10mg\u003cbr\u003e\nFormulation: Each vial contains Streptavidin lyophilized in sodium chloride buffer.\u003cbr\u003e\nPreparation: Reconstitute the content of the vial in distilled water to the desired concentration.\u003cbr\u003e\nStorage \u0026amp; Handling: For long-term storage, store the lyophilized streptavidin at -20°C or -70°C. After reconstitution, store the solution between 2°C and 8°C for up to 2 weeks, or store in aliquots at -20°C for up to 6 months.\u003cbr\u003e\nApplication: FC - Quality tested ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Nicosia M, et al. 2023. J Leukoc Biol. 544:113. PubMed Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed Urlaub D, et al. 2020. Curr Protoc Immunol. 131:e111. PubMed Ridge LA, et al. 2021. Cell Rep. 36:109610. PubMed\u003cbr\u003e\nStructure: 52 kD tetrameric protein\u003cbr\u003e\nDistribution: Streptavidin binds to biotin with high affinity\u003cbr\u003e\nLigand\/Receptor: Biotin\u003cbr\u003e\nAntigen References: 1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343. 2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093. 3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135. 4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113. 5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Biotin on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Streptavidin-PURE, SAV-PU, SAV\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866502221993,"sku":"405151","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-405151","provider":"Iright","version":"1.0","type":"link"}